轻工学报2025，Vol.40Issue(2)：72-79,8.DOI:10.12187/2025.02.008
以碳量子点为荧光信号的生物传感器构建及其在金银花Pb2+检测中的应用
The construction of biosensor with carbon quantum dots as fluorescence signal and its application in detecting Pb2+in honeysuckle
摘要
Abstract
Carbon quantum dots(G-CQDs)were prepared by hydrothermal method using grapefruit peel and fluorescence biosensor for Pb2+detection was constructed in honeysuckle.The morphology,structure and optical properties of G-CODs were characterized by circular dichroism spectroscopy,transmission electron microscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.The experimental conditions of the fluorescent biosensor were optimized and its detection performance was analyzed.The results showed that the G-CQDs were spherical and uniformly distributed.The average particle size was 2.41 nm,and the optimal excitation wavelength was 350 nm.The suitable experimental conditions for the fluorescence biosensor were as follows:Hemin concentration of 60 μmol/L,reaction time of 30 min,reaction temperature of 25℃,K+concentration of 10 mmol/L and pH value of 6.5.Compared with other metal ions(Ag+,Zn2+,Cd2+,Cu2+and Hg2+),the fluorescence biosensor had higher selectivity for Pb2+,and its fluorescence intensity increased with the increase of Pb2+mass concentration.In the linear range of 0.1～5.0 μg/mL,the linear relationship between the two was good,R2 was 0.998 0,and the detection limit was 0.063 9 μg/mL.Compared with the conventional method(ICP-MS method),the fluorescence biosensor had good accuracy,and had the advantages of green environmental protection,economical and effective,simple operation and so on.关键词
荧光生物传感器/碳量子点/DNAzyme/G-四链体/金银花/Pb2+Key words
fluorescent biosensor/carbon quantum dot/DNAzyme/G-quadruplex/honeysuckle/Pb2+分类
轻工纺织引用本文复制引用
李浩佳,贺诗华,曹艺泽,郭西玉,朱由余,赵玮钦,黄淳..以碳量子点为荧光信号的生物传感器构建及其在金银花Pb2+检测中的应用[J].轻工学报,2025,40(2):72-79,8.基金项目
国家自然科学基金青年基金资助项目(52102051) （52102051）
