以碳量子点为荧光信号的生物传感器构建及其在金银花Pb2+检测中的应用OA北大核心
The construction of biosensor with carbon quantum dots as fluorescence signal and its application in detecting Pb2+in honeysuckle
以葡萄柚皮为原料,采用水热法制备碳量子点(G-CQDs),并以此构建一种用于金银花中Pb2+检测的荧光生物传感器.利用圆二色光谱、透射电子显微镜、紫外-可见吸收光谱、荧光光谱等对G-CODs的形貌和光学性能进行表征,优化该荧光生物传感器的实验条件,并对其检测性能进行分析.结果表明:G-CQDs呈球形且分布均匀,平均粒径为2.41 nm,最佳激发波长为 350 nm;荧光生物传感器的适宜实验条件为Hemin浓度60 μmol/L、反应时间 30 min、反应温度 25℃、K+浓度 10 mmol/L 和 pH 值为 6.5;与其他金属离子(Ag+、Zn2+、Cd2+、Cu2+和Hg2+)相比,荧光生物传感器对Pb2+的选择性更高,且其Δ荧光强度随着Pb2+质量浓度的增加而增强,在0.1～5.0 μg/mL线性范围内,二者线性关系良好,R2 为 0.998 0,检测限为 0.063 9 μg/mL;与传统方法相比,该荧光生物传感器的准确性良好,且具有绿色环保、经济有效、操作简单等优势.
Carbon quantum dots(G-CQDs)were prepared by hydrothermal method using grapefruit peel and fluorescence biosensor for Pb2+detection was constructed in honeysuckle.The morphology,structure and optical properties of G-CODs were characterized by circular dichroism spectroscopy,transmission electron microscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.The experimental conditions of the fluorescent biosensor were optimized and its detection performance was analyzed.The results showed that the G-CQDs were spherical and uniformly distributed.The average particle size was 2.41 nm,and the optimal excitation wavelength was 350 nm.The suitable experimental conditions for the fluorescence biosensor were as follows:Hemin concentration of 60 μmol/L,reaction time of 30 min,reaction temperature of 25℃,K+concentration of 10 mmol/L and pH value of 6.5.Compared with other metal ions(Ag+,Zn2+,Cd2+,Cu2+and Hg2+),the fluorescence biosensor had higher selectivity for Pb2+,and its fluorescence intensity increased with the increase of Pb2+mass concentration.In the linear range of 0.1～5.0 μg/mL,the linear relationship between the two was good,R2 was 0.998 0,and the detection limit was 0.063 9 μg/mL.Compared with the conventional method(ICP-MS method),the fluorescence biosensor had good accuracy,and had the advantages of green environmental protection,economical and effective,simple operation and so on.
李浩佳;贺诗华;曹艺泽;郭西玉;朱由余;赵玮钦;黄淳
西安科技大学 化学与化工学院,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054||国土资源部煤炭资源勘查与综合利用重点实验室,陕西 西安 710054西安科技大学 化学与化工学院,陕西 西安 710054
轻工业
荧光生物传感器碳量子点DNAzymeG-四链体金银花Pb2+
fluorescent biosensorcarbon quantum dotDNAzymeG-quadruplexhoneysucklePb2+
《轻工学报》 2025 (2)
72-79,8
国家自然科学基金青年基金资助项目(52102051)
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