水杨酸诱导烟草黄酮类化合物合成基因表达研究OA北大核心

To explore the effects of salicylic acid(SA)on the total flavonoid content of tobacco(Nicotiana tabacum L.)and the expression of flavonoid synthesis-related genes,SA was applied to the one-month tobacco seedlings for 0 h,1 h,4 h and 12 h,and the total flavonoid content of the whole tobacco plant was detected and transcriptomic analysis was performed.The results of transcriptome analysis were verified by fluorescence quantitative PCR.The experimental results showed as follows:Compared with 0 h treated sample(SA0),the total flavonoid content of 1 h treated sample(SA1)was significantly up-regulated,and higher than that of 4 h treated sample(SA4)and 12 h treated sample(SA12).Analysis of differentially expressed genes(DEGs)showed that 1272 overlapping genes among SA1,SA4 and SA12 were significantly upregulated by SA treatment,GO functional annotation and KEGG metabolic pathway enrichment dispalyed that most of the genes significantly upregulated in SA1 encoded catalytic enzymes related to phenylpropanoid metabolism and isoflavone metabolism pathway,which could promote the biosynthesis of flavonoids such as lignin and its derivatives,flavins and anthocyanins.Fluorescence quantitative PCR analysis showed that the relative expression changes of differential genes were consistent with the corresponding differential gene expression trends in transcriptome analysis.This study provides clues and basis for seeking the genes involved in SA-induced flavonoids synthesis in tobacco and elucidating the regulatory network between SA and flavonoid biosynthetic pathway.