轻工学报2025，Vol.40Issue(2)：100-107,8.DOI:10.12187/2025.02.011
烟草肠杆菌芳香族氨基酸转氨酶的结构预测、克隆表达及酶学性质研究
Structural prediction,cloning,expression and enzymatic propertity of aromatic amino acid transaminase from Enterobacter tabaci
摘要
Abstract
To enhance the potential application of aromatic amino acid transaminase(AAT)in the synthesis of auxin(IAA)and promotion of crop growth,bioinformatics methods were applied to analyze and predict the structure of AAT from Enterobacter tabaci strain β7.Then,this AAT was cloned,expressed and purified in Escherichia coli,and the enzymatic properties of recombinant AAT were determined.The bioinformatics analysis results revealed that the length of this AAT protein is composed of 396 amino acids.The key residue in the active center of this AAT was Lys246.The molecular weight of the recombinant AAT expressed in E.coli was approximately 43 kDa.The optimal substrate for recombinant AAT was tryptophan,at a temperature of 50℃and a pH value of 8.0.Cu2+had a certain activating effect on enzyme activity,and recombinant AAT showed good tolerance to the organic solvent ethanol.These results provided a theoretical basis for the AAT enzyme modification and the construction of high-yield auxin engineering bacteria.关键词
烟草肠杆菌/芳香族氨基酸转氨酶/生长素/结构预测/克隆表达/酶学性质Key words
Enterobacter tabaci/aromatic amino acid transaminase/auxin/structural prediction/cloning and expression/enzymatic property分类
轻工纺织引用本文复制引用
徐瑞敏,邵化,李晨飞,单明明,魏涛..烟草肠杆菌芳香族氨基酸转氨酶的结构预测、克隆表达及酶学性质研究[J].轻工学报,2025,40(2):100-107,8.基金项目
中原科技创新人才项目(224200510017) （224200510017）
河南省重点研发专项项目(231111111700) （231111111700）
