基于HS-GC-IMS的不同部位及成熟度烟叶挥发性成分差异分析OA北大核心

To investigate the differences in volatile components of tobacco leaves from Yongzhou,Hunan,headspace-gas chromatography-ion mobility spectrometry(HS-GC-IMS)was employed to analyze volatile components in tobacco leaves with various parts and maturity levels.Differences of relative content were evaluated using principal component analysis,hierarchical cluster analysis,and orthogonal partial least squares regression analysis.The results showed a total of 69 volatile components were identified from tobacco leaves of different parts and maturity levels.Additionally,noticeable variations were observed in the volatile components among different tobacco leaves,with clear regional affiliations.The total 2b distinct volatile components were identified.Among these,the relative concentrations of 2-hexanone,isoamyl formate,furfural,2,6-dimethylpyrazine,2-methoxy-3-isobutylpyrazine,ethyl acetate,and geraniol exhibited significant variation in tobacco leaves depending on their stark positions within the plant.Concurrently,the relative concentrations of tetrahydrothiophene,2-hexanone,isoamyl formate,nonanal,acetic acid,indole,salicylaldehyde,and isobutanol showed significant variation in tobacco leaves at different stages of maturity.These findings could be used to distinguish tobacco leaves from different parts and maturity stages.