食品科学2025，Vol.46Issue(9)：30-38,9.DOI:10.7506/spkx1002-6630-20240625-181
乳酸乳球菌Q13细菌素对耐甲氧西林金黄色葡萄球菌的抑菌机理
Antibacterial Mechanism of Bacteriocin of Lactococcus lactis Q13 against Methicillin-Resistant Staphylococcus aureus
摘要
Abstract
The antibacterial mechanism of bacteriocin produced by Lactococcus lactis Q13 against methicillin-resistant Staphylococcus aureus(MRSA)was investigated in this study.The minimum inhibitory concentration(MIC)of the bacteriocin was tested,and its effects on bacterial growth,biofilm formation and DNA replication were evaluated.Meanwhile,quantitative polymerase chain reaction(qPCR)was used to study the effect of the bacteriocin on the expression of biofilm formation-related genes,virulence genes and genes related to DNA replication in MRSA.The results showed that bacteriocin Q13 could inhibit the growth of MRSA,with an MIC of 0.78 mg/mL.Scanning electron microscopy showed that bacteriocin Q13 could destroy the cell membrane of MRSA,leading to the leakage of intracellular contents.The leakage of nucleic acid and protein from MRSA cells increased obviously at all concentrations of bacteriocin Q13.At 1 MIC concentration,the leakage of nucleic acid and protein increased by 614.71％and 1 173.91％,respectively.The bacteriocin could inhibit and eradicate the biofilm of MRSA.At 1 MIC concentration,the inhibition rate and eradication rate of MRSA biofilm were 95.03％and 56.14％,respectively.Both synergistic and antagonistic effects were observed between antibiotics and bacteriocin Q13.The DNA content of MRSA cells decreased after treatment with the bacteriocin,and the expression of drug resistance genes,membrane formation-related genes,virulence genes and DNA replication-related genes was down-regulated.This study showed the potential of bacteriocin Q13 as a natural preservative in the food industry.关键词
乳酸乳球菌/细菌素/耐甲氧西林金黄色葡萄球菌/抑菌机制Key words
Lactococcus lactis/bacteriocin/methicillin-resistant Staphylococcus aureus/antibacterial mechanism分类
轻工业引用本文复制引用
刘爽,王金丽,梁安健,杨洋洋,朱成林,唐俊妮..乳酸乳球菌Q13细菌素对耐甲氧西林金黄色葡萄球菌的抑菌机理[J].食品科学,2025,46(9):30-38,9.基金项目
四川省自然科学基金面上项目(2024NSFSC0364) （2024NSFSC0364）
西南民族大学中央高校基本科研业务费专项资金项目(ZYN2025119) （ZYN2025119）
