食品科学2025，Vol.46Issue(9)：130-138,9.DOI:10.7506/spkx1002-6630-20240805-042
嗜热多聚半乳糖醛酸酶AfPGA性质表征及其在果胶低聚糖制备中的应用
Characterization of Thermophilic Polygalacturonase AfPGA and Its Application in the Preparation of Pectin Oligosaccharides
摘要
Abstract
Polygalacturonases(PGA)have important application prospects in the field of food processing.This study aims to heterologously express and characterize the thermophilic polygalacturonase AfPGA from Aspergillus fumigatus HBFH5 and explore its application for the preparation of pectin oligosaccharides(POS)form pineapple peel.The results showed that the polygalacturonase AfPGA belongs to the GH28 family,with a molecular mass of 39.2 kDa and an optimal temperature of 70℃.After treatment at 55℃for 1 h,the enzyme's activity remained stable.The optimal pH value was 5.0,and over 80％of the initial activity remained after treatment for 1 hour within the pH range of 2.0-12.0.It had strong resistance to various metal ions and chemical reagents.When using polygalacturonic acid as the substrate,the Km and vmax of the recombinant enzyme were 1.05 mg/mL and 52.6 μmol/(min·mg),respectively.Molecular dynamics(MD)simulation analysis found that the root mean square deviation(RMSD)and root mean square fluctuation(RMSF)of the mesophilic polygalacturonase ADPG2 were higher than those of AfPGA.In addition,POS prepared with AfPGA had strong antibacterial activity,which could significantly inhibit the growth of Salmonella,Staphylococcus aureus,Escherichia coli,and Shigella.POS also had good antioxidant properties.In summary,this study enriches the resources of thermophilic PGA,providing theoretical support for the application of this enzyme in POS preparation.关键词
多聚半乳糖醛酸酶/嗜热酶/分子动力学模拟/果胶低聚糖/抑菌活性/抗氧化性Key words
polygalacturonase/thermophilic enzyme/molecular dynamics simulation/pectin oligosaccharides/antibacterial activity/antioxidant activity分类
生物科学引用本文复制引用
郑辰,寇田田,谷新晰,刘学,田洪涛,卢海强..嗜热多聚半乳糖醛酸酶AfPGA性质表征及其在果胶低聚糖制备中的应用[J].食品科学,2025,46(9):130-138,9.基金项目
国家自然科学基金青年科学基金项目(31901631) （31901631）
