食品科学2025，Vol.46Issue(9)：303-313,11.DOI:10.7506/spkx1002-6630-20241125-169
基于超临界流体色谱-串联质谱法同时测定乳液中α,β-不饱和醛
Simultaneous Determination of α,β-Unsaturated Aldehydes in Emulsion Using Supercritical Fluid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
In order to effectively improve the detection of α,β-unsaturated aldehydes in emulsion,this study established an efficient and sensitive detection method using supercritical fluid chromatography-tandem mass spectrometry(SFC-MS/MS).Sample pretreatment conditions and SFC-MS/MS parameters were optimized.It was found that the simultaneous detection of eight α,β-unsaturated aldehydes was achieved within 4 minutes using a mobile phase composed of supercritical CO2 fluid and acetonitrile under the negative ionization mode.The α,β-unsaturated aldehydes showed good linearity over their respective concentration ranges,limits of quantification(LOQ)lower than 1.8 μg/kg and spiked recoveries between 85.8％and 106.7％.The relative standard deviations(RSD)of accuracy were lower than 7.86％.The optimized pretreatment conditions were as follows:1.25 mg of 2,4-dinitrophenylhydrazine,derivatization time of 30 minutes,four cycles of extraction with acetonitrile,and three cycles of re-extraction with dichloromethane.Furthermore,the established method was used to evaluate the effects of three polyphenolic antioxidants:water-soluble gallic acid,water-oil amphipathic propyl gallate and water-and oil-insoluble quercetin on reducing the eight α,β-unsaturated aldehydes in linseed oil-in-water emulsion during storage.The results showed that α,β-unsaturated aldehydes accumulated rapidly during the storage of the emulsion,mainly malondialdehyde,4-hydroxy-2-hexenal and 4-hydroxy-2-nonenal,while all three antioxidants significantly mitigated the accumulation of α,β-unsaturated aldehydes,with propyl gallate being the most effective.This method is a simple,efficient,environment-friendly,sensitive,and accurate one for the detection of α,β-unsaturated aldehydes generated during the processing,storage,and use of emulsion-based foods.关键词
超临界流体色谱-串联质谱/乳液/亚麻籽油/油脂氧化/α,β-不饱和醛Key words
supercritical fluid chromatography-tandem mass spectrometry/emulsions/flaxseed oil/lipid oxidation/α,β-unsaturated aldehydes分类
轻工业引用本文复制引用
王曙,魏世肖,张浩,孙尚德,毕艳兰..基于超临界流体色谱-串联质谱法同时测定乳液中α,β-不饱和醛[J].食品科学,2025,46(9):303-313,11.基金项目
国家自然科学基金青年科学基金项目(32202198) （32202198）
河南省科技研发计划联合基金项目(242103810086) （242103810086）
郑州市科技局自然科学项目(22ZZRDZX23) （22ZZRDZX23）
