食品与机械2025，Vol.41Issue(3)：39-44,6.DOI:10.13652/j.spjx.1003.5788.2024.60144
基于量子点纳米粒子对乳制品中致病菌进行定性分析
Qualitative analysis of pathogenic bacteria in dairy products based on quantum dot nanoparticles
摘要
Abstract
[Objective]To develop a novel multivariate qualitative analysis method for detecting pathogenic bacteria in food microorganisms based on quantum dot nanoparticles,aiming to meet the current requirements of food safety supervision and the timeliness of short-shelf-life food detection.[Methods]Quantum dots,with their diverse categories and various synthesis methods,were combined with immunofluorescence analysis to establish a rapid,sensitive,and efficient method for detecting two or more different pathogenic bacteria.[Results]This method enabled the simultaneous,rapid,sensitive,and efficient detection of foodborne pathogens.When the bacterial concentration was 103 CFU/mL,pathogens could still be detected within 15 min.Blind sample testing of pathogenic bacteria in dairy products with different substrates showed a 100%agreement rate with traditional methods.[Conclusion]This method exhibits strong specificity and high sensitivity for pathogen detection,while also being simple to operate and requiring a short detection time,making it highly practical.关键词
量子点/纳米粒子/乳制品/致病菌/定性分析Key words
quantum dot/nanoparticles/dairy products/pathogenic bacterium/qualitative research引用本文复制引用
王芳妹,方群,钟文涛,李政,徐越,苏家佑,向俊,陈瑶,李白玉,卢琳..基于量子点纳米粒子对乳制品中致病菌进行定性分析[J].食品与机械,2025,41(3):39-44,6.基金项目
湖南省市场监督管理局科技计划项目(编号:2024KJJH07,2023KJJH16) （编号:2024KJJH07,2023KJJH16）
