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基于香豆素类荧光探针可视化检测食品中氰化物

胡小军 李政 汪霞丽 张新 何青科 刘湘

食品与机械2025,Vol.41Issue(3):45-49,5.
食品与机械2025,Vol.41Issue(3):45-49,5.DOI:10.13652/j.spjx.1003.5788.2024.60123

基于香豆素类荧光探针可视化检测食品中氰化物

Visual detection of cyanide in food based on coumarin fluorescent probe

胡小军 1李政 1汪霞丽 1张新 1何青科 1刘湘2

作者信息

  • 1. 湖南省产商品质量检验研究院,湖南 长沙 410117
  • 2. 湖南师范大学,湖南 长沙 410000
  • 折叠

摘要

Abstract

[Objective]To develop a method for detecting cyanide in food based on a novel fluorescent probe.[Methods]Based on the strong nucleophilicity of cyanide,a novel reactive coumarin fluorescent probe is synthesized.The response mechanism,reaction rate,selectivity,and linear relationship between the probe and cyanide are analyzed and validated using real samples.[Results]The probe has excellent selectivity for cyanide and demonstrates a rapid response to cyanide,able to complete the reaction within 1 minute.At a probe concentration of 10 µmol/L,the linear range of cyanide detection is 5~90 µmol/L,with a linear equation of Y=11.645 45+7.014 55X and a detection limit of 0.065 µmol/L.In actual sample testing,the recovery rate of spiked samples is 97.9%~105.0%.[Conclusion]This probe can quickly and accurately detect cyanide.

关键词

荧光探针/食品/氰化物/香豆素

Key words

fluorescent probe/food/cyanide/coumarin

引用本文复制引用

胡小军,李政,汪霞丽,张新,何青科,刘湘..基于香豆素类荧光探针可视化检测食品中氰化物[J].食品与机械,2025,41(3):45-49,5.

基金项目

湖南省市场监督管理局科技计划项目(编号:2024KJJH12) (编号:2024KJJH12)

食品与机械

OA北大核心

1003-5788

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