食品与机械2025，Vol.41Issue(3)：161-170,10.DOI:10.13652/j.spjx.1003.5788.2024.80701
酒糟多酚类物质纯化及体外抗氧化活性研究
Purification of polyphenols from distiller's grains and its antioxidant activity in vitro
晏俊玲 1郑佳 1张伟建 1李茂 1赵佳伟 1覃凤阳1
作者信息
- 1. 宜宾五粮液股份有限公司技术研究中心,四川 宜宾 644007||中国轻工业浓香型白酒固态发酵重点实验室,四川 宜宾 644000
- 折叠
摘要
Abstract
[Objective]To purify and enrich the distiller's grain polyphenols(DGPs)with good antioxidant activity.[Methods]Organic solvent extraction combined with macroporous adsorption resin was used to enrich and recover DGPs from the distiller's grains deep eutectic solvent(DES)extraction solution.The purity and antioxidant activity of the purified products were determined.Extraction solvents and resin types were screened,and adsorption kinetics,isotherms,and thermodynamics were used to explain the adsorption mechanism.The optimal process conditions were determined.The in vitro antioxidant activity of the polyphenols was evaluated by measuring their ability to scavenge 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)free radicals and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt(ABTS)free radicals.[Results]Dichloromethane was the most suitable extraction solvent,with a polyphenol recovery rate of 90.83%when the ratio of dichloromethane to DES was 2∶1.The best resin for purifying DGPs was DM130,with an adsorption rate of 78.70%.The adsorption of DGPs by DM130 resin conformed to pseudo-second-order kinetics and the Langmuir adsorption model,indicating that the process is spontaneous and exothermic.The optimal purification conditions with DM130 resin were a sample solution pH of 5,a sample concentration of 0.8 mg/mL,a flow rate of 2 BV/h,a sample volume of 3.4 BV,and elution with 60%ethanol at a flow rate of 3 BV/h and an elution volume of 6.9 BV.The overall recovery rate of DGPs from the distiller's grains DES extraction solution after dichloromethane extraction and DM130 resin adsorption was 88.90%,with polyphenol purity increasing from 2.11%to 16.05%.Seven phenolic compounds were detected in the purified product,with ferulic acid being the most abundant.The IC50 values for scavenging DPPH and ABTS+free radicals were 0.080 4 mg/mL and 0.185 0 mg/mL,respectively.[Conclusion]The purification method for the DGPs established in this study is effective and feasible,and the enriched DGPs exhibit good antioxidant activity.关键词
酒糟多酚/大孔吸附树脂/分离纯化/机理/抗氧化活性Key words
distiller's grain polyphenols(DGPs)/macroporous adsorption resin/separation and purification/mechanism/antioxidant activity引用本文复制引用
晏俊玲,郑佳,张伟建,李茂,赵佳伟,覃凤阳..酒糟多酚类物质纯化及体外抗氧化活性研究[J].食品与机械,2025,41(3):161-170,10.
