沈阳农业大学学报2025，Vol.56Issue(2)：34-41,8.DOI:10.3969/j.issn.1000-1700.2025.02.004
水稻纹枯病抗性相关基因OsDUF功能研究
Functional Study of the Rice Sheath Blight Resistance Related Gene OsDUF
摘要
Abstract
[Objective]Rice sheath blight is one of the major diseases in rice.Due to the wide host range of Rhizoctonia solani and the lack of resistant rice resources,it causes serious losses of rice production.Previous study,we investigated the roles of rice sheath blight-responsive hormones and the candidate resistance gene OsDUF,providing the theoretical basis for the prevention and controlling rice sheath blight.[Methods]The susceptible variety Yanfeng 47 and the resistant rice variety Gangyuan 8 were selected for inoculation with the rice sheath blight fungus to identify the disease resistant candidate gene OsDUF,bioinformatics analysis and subcellular localization of OsDUF were conducted.Resistance of CRISPR-Cas9-generated OsDUF mutants were compared to wild-type plants.Yeast two-hybrid assays were performed using OsDUF as bait protein,and changes in the expression levels of OsDUF genes were detected under exogenous hormonal treatments,furthermore changes in the expression levels of defence genes and hormone-related genes were detected under treatment with R.solani.[Results]Phylogenetic characterization revealed that OsDUF,encoding a cytoplasm-localized protein possessing the DUF1645 domain characteristic of stress-responsive proteins,exhibits closest evolutionary relationship to japonica rice accessions(Oryza sativa subsp.japonica).Bioinformatics interrogation further identified ten putative rice interactors through domain architecture analysis,though subsequent experimental validation remains required.Identification of resistance in isolated leaves showed that the mutant was more susceptible to the disease than the wild type,indicating that OsDUF positively regulates rice sheath blight resistance,and one-to-one interaction validation did not reveal that OsDUF interacted with the predicted candidate proteins.Under exogenous hormone treatments,the genes OsDUF were up-regulated by indoleacetic acid,ethylene,and jasmonic acid,and down-regulated by gibberellin,abscisic acid,and salicylic acid.After inoculation with R.solani,the expression levels of defence related genes(OsAOS1,OsPAL1)and hormone-related genes(OsERF063,LOX2)were significantly higher in the wild type than in the mutant.[Conclusion]The results suggest that OsDUF may be involved in the resistance of rice to R.solani through the way of modulation of defence genes and hormone signaling pathways.关键词
水稻纹枯病/OsDUF/生物信息学分析/激素Key words
rice sheath blight/OsDUF/bioinformatics analysis/hormone分类
植物保护学引用本文复制引用
王妍,王梦雨,项宗敬,王奕鸣,魏松红..水稻纹枯病抗性相关基因OsDUF功能研究[J].沈阳农业大学学报,2025,56(2):34-41,8.基金项目
国家重点研发计划项目(2024YFD1201005-05) （2024YFD1201005-05）
国家水稻产业技术体系项目(CARS-01) （CARS-01）
"兴辽英才计划"农业专家项目(XLYC2213046) （XLYC2213046）
