不同免疫增强剂对猫瘟疫苗免疫效力的影响OA北大核心

[Objective]Feline distemper is an infectious disease of cats caused by feline microvirus,which is mainly characterised by decreased leukocyte counts and haemorrhagic gastroenteritis,and has a high rate of infection and lethality,posing a serious threat to the health of pets.Vaccination is currently the most important way to prevent feline distemper,but the immune effect of the vaccine is constrained by a variety of factors.Immunity enhancers can enhance the effect of vaccine immunity in livestock and poultry.We explored the effect of immunity enhancers on the effect of vaccine immunity in pets,aiming to provide a theoretical basis for the effect of immunity prevention and control in cats.[Methods]Young cats were selected as experimental subjects.Based on a one-way experimental design,each experimental group was immunised with Myo-Santo vaccine at 60,81 and 102 days of age,and fed with 0.9%NaCl saline(control group),Astragali polysaccharides(APC group),splenic aminotransferase peptide(SAT group),and high immunity factor nucleic acid preparations(NAP group),respectively,for 4 d before and after immunization.All groups were examined for their IgG antibody,IFN-γ and whole blood cell classification were detected.[Results]Splenic aminopeptide and high immunity factor nucleic acid preparation were able to increase the secretion levels of distemper antibody and INF-γ,as well as the number of leukocytes and neutrophils in young cats,but astragali polysaccharides did not have a significant effect on the secretion levels of distemper antibody and INF-γ in young cats;and splenic aminopeptide and high immunity factor nucleic acid preparation did not have a significant effect on the number of erythrocytes,platelets,monocytes,and lymphocytes in young cats.[Conclusion]Splenic aminopeptide and high immunity factor nucleic acid preparations were able to significantly increase the levels of some antibody indexes in young cats,suggesting that these two reagents can improve the immunity effect of young cats against feline distemper.This study lays a preliminary theoretical foundation for the study of immune enhancers in young cats,accumulates data for further research on the effective prevention and treatment of feline distemper and other diseases,and provides a reference for the use of common immune-enhancing reagents for poultry and livestock to enhance the immunity of young cats and other pets.