高温胁迫下柞蚕抗氧化酶活性分析OA北大核心

[Objective]With the global warming,the average temperature in Northeast China has also increased year by year.The high ambient temperature is not conducive to the growth and development of Antheraea pernyi larvae,causing serious economic losses to the A.pernyi industry.[Methods]This experiment studied the changes of SOD,CAT and POD activities in the larvae of six A.pernyi varieties under 42 ℃ high temperature stress,analyzed the heat resistance of six A.pernyi varieties,and screened out high-quality heat-resistant A.pernyi resources.[Results]The results showed that the antioxidant enzyme activities of the six A.pernyi varieties increased first and then decreased with the increase of high temperature stress time.Except for the peak of Xiaobaican appeared at 2-3 h,the peak of other varieties appeared at 4-5 h,indicating that the response of Xiaobaican to high temperature stress was weaker than that of other varieties,and there were significant differences between the stress group and the control group of the same variety.There were significant differences in CAT activity between Fangshanhuang,F,Yufeng,Xiaobaican and Qing 6(P<0.05).There were dominant differences in POD activity between Fangshanhuang,Xiaobaican and Qing 6(P<0.05).There were significant differences in SOD activity between Fangshanhuang,Jiufeng,Xiaobaican and Qing 6(P<0.05).Comprehensive evaluation of the heat resistance of each variety,with Qing 6 as the control,Fangshanhuang,F,Jiufeng and Yufeng showed strong heat resistance,while the heat resistance of Xiaobaican was relatively weak.[Conclusion]The research results can provide a theoretical basis for the study of heat resistance mechanism of A.pernyi and the screening of heat-resistant germplasm resources.