中国果菜2025，Vol.45Issue(4)：35-40,6.DOI:10.19590/j.cnki.1008-1038.2025.04.006
猕猴桃根部源分散泛菌葡萄糖异构酶异源表达研究
Study on Heterologous Expression of Glucose Isomerase from Pantoea dispersa of Kiwifruit Root Origin
摘要
Abstract
To address the limitations of traditional high-fructose syrup production,such as low catalytic efficiency of conventional glucose isomerases,this study focused on the heterologous expression and optimization of a novel glucose isomerase,Pd-xylA,derived from Pantoea dispersa isolated from kiwifruit roots.The Pd-xylA gene was cloned and expressed in Escherichia coli BL21(DE3),followed by systematic optimization of induction conditions including temperature and IPTG(isopropyl-β-D-thiogalactopyranoside)concentration.The results demonstrated that the optimal induction conditions were 28℃and 0.1 mmol/L IPTG,under which Pd-xylA achieved the highest soluble expression level.This study provided theoretical support for the development of enzyme resources with enhanced catalytic activity and industrial applicability,thereby advancing the biotechnological production of high-fructose syrup.关键词
分散泛菌/葡萄糖异构酶/诱导条件优化/异源表达Key words
Pantoea dispersa/glucose isomerase/optimization of induction conditions/heterologous expression分类
物理学引用本文复制引用
智勇,夏菠..猕猴桃根部源分散泛菌葡萄糖异构酶异源表达研究[J].中国果菜,2025,45(4):35-40,6.基金项目
湖南省自然科学基金项目(2022JJ30299、2022JJ30290) （2022JJ30299、2022JJ30290）
