中国食品添加剂2025，Vol.36Issue(4)：66-75,10.DOI:10.19804/j.issn1006-2513.2025.4.010
火麻脱脂蛋白酶解工艺优化及其多肽降血糖活性研究
Optimization of hemp defatted protease hydrolysis process and the hypoglycemic activity of its peptides
摘要
Abstract
Hemp defatted protein was used as the raw material,with the inhibition rate of Dipeptidyl Peptidase Ⅳ(DPP-Ⅳ)as the evaluation criteria,neutral protease was employed for the hydrolysis to prepare hemp peptides.Based on single factor experiments,the enzymatic hydrolysis process of hemp peptides was optimized using response surface methodology.The results showed that the optimal enzymatic hydrolysis process of hemp defatted protein was:a material to liquid ratio of 1:8 g/mL,enzyme addition of 5.7%,and enzymatic hydrolysis time of 6 hours.Under these conditions,the inhibition rate of the DPP-Ⅳ by the hydrolysis product reached 38.24%.The hydrolysis product was fractionated into four components of different molecular weights by ultrafiltration,among which the peptides with a molecular weight of less than 1 kDa exhibited the strongest hypoglycemic activity,with DPP-Ⅳ inhibition rates of 41.06%,α-amylase inhibition rates of 64.79%,and α-glucosidase inhibition rates of 48.51%.This study lays the foundation for the further separation and purification of hypoglycemic peptides from hemp defatted protein and demonstrates certain research application value in the development of therapeutic special medical foods for diabetes.关键词
火麻蛋白/二肽基肽酶-Ⅳ/多肽/工艺优化Key words
hemp protein/dipeptidyl peptidase Ⅳ/peptides/process optimization分类
轻工业引用本文复制引用
杨策,李会珍,张志军,侯天宇,况丹妮..火麻脱脂蛋白酶解工艺优化及其多肽降血糖活性研究[J].中国食品添加剂,2025,36(4):66-75,10.基金项目
山西省重点研发计划项目特优农业技术创新专项(2022ZDYF123) （2022ZDYF123）
山西省科技成果转化引导专项(202204021301046) （202204021301046）
山西省基础研究计划青年项目(202203021222032) （202203021222032）
