食品工业科技2025，Vol.46Issue(9)：165-175,11.DOI:10.13386/j.issn1002-0306.2024050348
虾壳粉添加量对小龙虾酱发酵过程中挥发性风味化合物及微生物群落动态变化的影响
Effects of Addition of Shrimp Shell Powder on Volatile Flavor Compounds and Dynamic Changes of Microbial Community during Fermentation of Crayfish Shrimp Paste
摘要
Abstract
This study focused on crayfish shrimp paste as the research subject,with the addition of 0,20％,and 40％shrimp shell powder,respectively.Across three fermentation stages of 0,6,and 12 days for the crayfish shrimp paste,an investigation into the flavor and microbial evolution was conducted utilizing electronic noses,gas chromatography-mass spectrometry(GC-MS),and 16S rRNA sequencing.The results showed that adding shrimp shell powder could significantly increase the types and contents of volatile flavor compounds(sulfur compounds,amines,ketones,esters and heterocycles)in shrimp paste samples,and adding 20％shrimp shell powder could better promote the production and growth of volatile flavor compounds.Combining odor activity value(OAV)and variable importance for the projection(VIP)screened out three substances(2-decanone,methylmercaptan,dimethyl trisulfide)as the signature flavor compounds to distinguish the difference of different addition amounts of shrimp shell powder and different fermentation time.The addition of shrimp shell powder increased the abundance and diversity of microbial genera(Oceanobacillus,Virgibacillus,Cerasibacillus).Combined with Spearman correlation coefficient analysis,it was found that the unique Virgibacillus in the shrimp shell powder sample group showed significant positive correlation with several key flavor compounds and contributed the most to flavor.In summary,adding shrimp shell powder to lobster paste,especially when the amount is 20％,is conducive to promoting the growth of flavor-related microorganisms and enriching the flavor of shrimp paste.关键词
小龙虾/虾壳粉/虾酱/挥发性风味化合物/微生物Key words
crayfish/shrimp shell powder/shrimp paste/Volatile flavor compounds/microbes分类
轻工业引用本文复制引用
于金玉,谌玲薇,胡传峰,陈戈辉,李弥友,乔宇,于巍..虾壳粉添加量对小龙虾酱发酵过程中挥发性风味化合物及微生物群落动态变化的影响[J].食品工业科技,2025,46(9):165-175,11.基金项目
湖北省科技创新人才计划-科技人才服务企业项目(2023DJC100) （2023DJC100）
潜江市公益性行业科研计划项目(2023GYX030). （2023GYX030）
