食品工业科技2025，Vol.46Issue(9)：185-195,11.DOI:10.13386/j.issn1002-0306.2024060069
后生元PostbioYDFF对铜绿假单胞菌的抑菌活性及其转录机制
Inhibitory Activity and Transcriptional Mechanism of PostbioYDFF against Pseudomonas aeruginosa
摘要
Abstract
In the present study,the antibacterial activity of postbiotics PostbioYDFF against Pseudomonas aeruginosa(P.aeruginosa)and its mechanism of action were examined by analyzing its effects on biofilm formation and changes in the ultrastructure and transcriptome.Meanwhile,to validate transcriptome data,qPCR was conducted.The results showed that Postbio YDFF exhibited significant bacteriostatic activity against P.aeruginosa with a diameter of inhibition zone growth of 28.9±0.3 mm and the MIC value of 100 uL/mL.In addition,compared with the control group,PostbioYDFF was able to significantly inhibit biofilm formation by 64.96％(P＜0.01)and disrupted established biofilms by 30.66％(P＜0.05).It was also observed that PostbioYDFF disrupted the bacterial structure and attenuated bacterial motility(swimming,swarming and twitching).Through RNA-seq analysis combined with qPCR validation,a total of 503 significantly different genes were found after PostbioYDFF treatment as compared with the control group,including 237 significantly up-regulated genes which were mainly enriched in ribosomal,RNA degradation,oxidative phosphorylation and other pathways,and 266 significantly down-regulated genes which were mainly enriched in the energy metabolism,lipid metabolism,and two-component system and other pathways.This study provides an important theoretical basis for the prevention and control of P.aeruginosa by PostbioYDFF,and points out a potential application in food preservation.关键词
铜绿假单胞菌/后生元/转录组学/抑菌/防腐Key words
Pseudomonas aeruginosa/postbiotics/transcriptome/bacteriostasis/antiseptic分类
轻工纺织引用本文复制引用
刘于倩,王铭,袁伟,臧金红,赵金山,李兆杰,胡瑞艳,彭传涛..后生元PostbioYDFF对铜绿假单胞菌的抑菌活性及其转录机制[J].食品工业科技,2025,46(9):185-195,11.基金项目
国家自然科学基金(32101917) （32101917）
青岛农业大学高层次引进人才项目(1122024). （1122024）
