食品工业科技2025，Vol.46Issue(9)：196-205,10.DOI:10.13386/j.issn1002-0306.2024060197
副干酪乳杆菌Lp.R3的高密度培养工艺优化
Optimization of High-density Culture Process of Lacticaseibacillus paracasei Lp.R3
摘要
Abstract
Lacticaseibacillus paracasei Lp.R3 is a probiotic strain with excellent functions.In order to realize its industrial application,high-density cultivation was carried out to increase the number of viable bacteria,the effects of medium composition,static culture conditions,and fermentation tank processes on the number of viable Lp.R3 bacteria were investigated by single factor experiments and orthogonal experiments optimization.The best result(4.75× 109 CFU/mL of viable bacteria number)was achieved when Lp.R3 cultured in the medium supplemented with 30 g/L glucose,50 g/L yeast extract,10 g/L anhydrous sodium acetate,0.1 g/L manganese sulfate,and 1 g/L Tween80 under the culture conditions of 1％inoculation level,pH7 and 35 ℃,which was 2.91 times greater than that cultured in MRS medium.In terms of the production in 5 L fermentation tanks,the best result(2.56× 1010 CFU/mL of viable bacteria number)was given when L.p R3 fermented under constant pH of 5 neutralized by 25％ammonia,continuous variable feed of 75 g/L glucose,and agitation without aeration,which was 15.51 times greater than that cultured in MRS medium.In summary,the high-density production protocol of Lp.R3 was optimized in present study,providing a promising guide to accomplish large-scale production of Lp.R3.关键词
副干酪乳杆菌/高密度培养/恒pH培养/工艺优化/活菌数Key words
Lacticaseibacillus paracasei/high-density culture/constant pH cultivation/process optimization/viable count分类
轻工纺织引用本文复制引用
方诗会,熊尧,张召,林俊芳,陈涛,郭丽琼..副干酪乳杆菌Lp.R3的高密度培养工艺优化[J].食品工业科技,2025,46(9):196-205,10.基金项目
广东省重点领域研发计划项目(2022B0202050002) （2022B0202050002）
国家自然科学基金项目(32072646,32272785) （32072646,32272785）
2021年度佛山市科技创新团队(青年拔尖人才)(2120001010795). （青年拔尖人才）
