食品工业科技2025，Vol.46Issue(10)：342-350,9.DOI:10.13386/j.issn1002-0306.2024050117
黄芪茎叶增强免疫力的活性部位与化学成分研究
Active Fractions with Immune-enhancing Effects from the Stems and Leaves of Astragalus membranaceusand Its Chemical Components Identification
摘要
Abstract
Objective:Active fractions with immunomodulatory effects from the stems and leaves of Astragalus membranaceus(AMSL)were investigated and its chemical constituents were analyzed.Methods:The immunosuppression mice models were established by injection of cyclophosphamide,and the effects of several AMSL extracts on the immune response of mice were investigated,including crude extract,petroleum ether fraction,chloroform fraction,ethyl acetate fraction,n-butanol fraction and water fraction.Meanwhile,the control group,model group,and positive group were included.Hematoxylin-eosin staining was used to examine the spleen tissue morphology of mice after 28 d of continuous administration,and the immune organ indexes,immune cell capabilities,and levels of immune components in each group were analyzed.Furthermore,the components in the active fraction of AMSL were identified through ultra-performance liquid chromatography/ion mobility quadrupole time-of-flight mass spectrometry(UPLC/IM-QTOF-MS).Results:The n-butanol fraction from AMSL was proved to show significant immunomodulatory activity.In comparison to the model group,the growth rate of body weight,immune organ indexes,splenic lymphocyte proliferation index,macrophage phagocytic capacity,delayed allergic reaction severity,and serum hemolysin level in mice were significantly enhanced(P<0.05)in the group treated by this active fraction.Besides,the serum levels of immunoglobulin A,interleukin-6,interferon-γ and tumor necrosis factor-α were also significantly increased by 39.96％,15.87％,16.22％and 47.68％respectively after the intervention of this fraction(P<0.05),which was revealed to be primarily composed of such substances as flavonoids,saponins,and phenolic acids elucidated by UPLC/IM-QTOF-MS.Conclusion:The active fraction with immunomodulatory effects from AMSL was the n-butanol fraction,which established a basis for further study on the immunoregulatory activity of AMSL.关键词
黄芪茎叶/免疫低下/环磷酰胺/增强免疫力/正丁醇部位Key words
the stems and leaves of Astragalus membranaceus(AMSL)/immune suppression/cyclophosphamide/enhancing immunity/n-butanol fraction分类
轻工业引用本文复制引用
高欣缘,李小兰,李思卿,李越,秦楠,郭丽丽..黄芪茎叶增强免疫力的活性部位与化学成分研究[J].食品工业科技,2025,46(10):342-350,9.基金项目
山西省自然科学研究面上项目(202303021221160) （202303021221160）
山西中医药大学科技创新能力培育计划"太行本草"专项项目(2022PY-TH-04) （2022PY-TH-04）
山西省科技创新人才团队专项资助(202304051001043) （202304051001043）
山西中医药大学校级科技创新团队(2022TD2008). （2022TD2008）
