食品工业科技2025，Vol.46Issue(9)：372-380,9.DOI:10.13386/j.issn1002-0306.2024050346
枳椇果梗多糖调节谷氨酸代谢和紧密连接蛋白表达改善酒精对小鼠神经行为影响
Hovenia dulcis Fruit Peduncle Polysaccharides Modulate Glutamate Metabolism and Tight Junction Protein Expressions to Attenuate the Neurotoxic Effects of Alcohol on Mice
摘要
Abstract
Objective:This study aimed to investigate the protective effects of Hovenia dulcis fruit peduncle polysaccharides(HDPs)on alcohol-induced neurobehavioral alterations in mice and to elucidate whether HDPs mitigate alcohol-induced neuronal damage by modulating glutamate metabolic pathway and the expression of tight junction protein.Methods:Male C57BL/6 mice were administered alcohol intragastrically at a dose of 114 μL/20 g for 14 d to establish an alcohol exposure model,and an intervention group was set up for HDPs intervention(114 μL/20 g alcohol+100 mg/kg HDPs).Behavioral experiments(open field test,elevated plus maze test)were used to assess changes in neurobehavior,gas chromatography was used to determine ethanol concentrations in mouse blood.γ-H2AX fluorescence was used to detect DNA damage in mouse hippocampal tissue,and immunohistochemical analysis was used to detect the expression of tight junction proteins Claudin-1 and ZO-1 in mouse brain tissue.Metabolites in mouse brain tissue were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)metabolomics technology.Results:HDPs effectively reduced blood ethanol concentration in alcohol-exposed mice(from 4.69±0.29 g/L to 1.64±0.104 g/L)and ameliorated alcohol-induced neurobehavioral abnormalities.In the open field test,compared to the alcohol group,HDPs intervention significantly increased total distance traveled(27340±3304 cm,P＜0.05)and average velocity(67.4±13.4 cm/s,P＜0.05),while reduced immobility time by 29％(P＜0.05).The elevated plus maze test revealed that HDPs treatment decreased closed-arm dwelling time(195.6±10.3 s,P＜0.05)and increased open-arm entries by 26％(P＜0.05)compared to the alcohol group.Additionally,HDPs alleviated alcohol-induced oxidative brain damage by reducing ROS levels(5.4％)and MDA content(29.5％)(P＜0.05)respectively,while enhanced total antioxidant capacity(T-AOC)by 10.9％.It upregulated hippocampal expression of tight junction proteins Claudin-1(2.2-fold)and ZO-1(10％).HDPs also modulated glutamatergic metabolic pathways,elevating brain levels of glycine,glutathione,and succinate by 19.7％,25％and 22.6％respectively.Conclusion:HDPs can effectively alleviate the neurobehavioral impact of alcohol on mice.The mechanism may involve antioxidant activity,protection of tight junction proteins,and modulation of glutamate metabolic pathways,providing a theoretical basis for the development and application of Hovenia dulcisis resources in the food field.关键词
枳椇果梗多糖(HDPs)/酒精/神经行为/谷氨酸代谢/紧密连接蛋白Key words
Hovenia dulcis fruit peduncle polysaccharides/alcohol/neurobehavior/glutamate metabolism/tight junction proteins分类
轻工纺织引用本文复制引用
张玉超,袁子飞,刘良禹,朱思洁,杨永,郑代梅,刘旭东..枳椇果梗多糖调节谷氨酸代谢和紧密连接蛋白表达改善酒精对小鼠神经行为影响[J].食品工业科技,2025,46(9):372-380,9.基金项目
贵州省基础研究(自然科学)计划项目(黔科合基础-ZK[2022]一般541,黔科合基础-ZK[2022]一般542) （自然科学）
遵义市科技计划项目(遵市科合HZ字(2024)387号) （遵市科合HZ字(2024）
茅台学院酒产业研究院2024年专项课题(MTXYJCY009) （MTXYJCY009）
茅台学院高层次人才科研启动经费项目(mygccrc[2022]010) （mygccrc[2022]010）
贵州省大学生创新创业训练计划项目(S202214625019,S202214625021). （S202214625019,S202214625021）
