食品工业科技2025，Vol.46Issue(10)：21-32,12.DOI:10.13386/j.issn1002-0306.2024090061
燕麦非淀粉多糖发酵富集法工艺优化及其免疫刺激活性分析
Optimization of the Fermentation Enrichment Method for Oat Non-starch Polysaccharides and Study of Their Immunostimulatory Activity
摘要
Abstract
In order to enhance the yield of oat non-starch polysaccharide(ONSP),the extraction process was optimized and its immunological activity was investigated.This study employed microbial fermentation-assisted extraction,optimizing the process for oat non-starch polysaccharide(ONSP1)using a one-way response surface methodology.Fourier transform infrared spectroscopy(FTIR)and gas chromatography-mass spectrometry(GC-MS)were employed to determine the structural characteristics of ONSP.Additionally,the immunological activity of ONSP was investigated and compared with that of oat non-starch polysaccharide(ONSP2)extracted through the conventional hot water method.The results demonstrated that the optimal extraction process for ONSP1 was achieved with a 5％of the inoculum and fermentation at 34℃for 26 h,resulting in a yield of ONSP1 at 8.60％±0.04％.Both ONSP1 and ONSP2 were composed of glucose,arabinose,and xylose,with the contents of arabinose and xylose of ONSP1 were significantly higher than those of ONSP2(P<0.05).Despite the lower molecular weight of ONSP1(47.2 kDa)compared to ONSP2(53.2 kDa),ONSP1 had a significantly higher β-glucan content(P<0.05).Furthermore,both ONSP1 and ONSP2 demonstrated the capacity to promote the proliferation of RAW264.7 cells,with ONSP1 exhibiting a more pronounced immune-stimulating activity.In conclusion,microbial fermentation enrichment resulted in oat non-starch polysaccharides with a higher β-glucan content and stronger immunostimulatory activity.This study provides new insights for further investigation into the bioimmunological activities and structural relationships of oat non-starch polysaccharides.关键词
燕麦非淀粉多糖/微生物发酵/工艺优化/结构表征/免疫刺激活性Key words
oat non-starch polysaccharides/microbial fermentation/process optimization/structural characterization/immunostimulatory activity分类
轻工纺织引用本文复制引用
高若涵,马楠,杨明哲,曹家宝,王霞,鹿保鑫..燕麦非淀粉多糖发酵富集法工艺优化及其免疫刺激活性分析[J].食品工业科技,2025,46(10):21-32,12.基金项目
国家重点研发计划项目"稻谷加工精准调控技术研究及营养平衡型开发与示范"(2021YFD2100902). （2021YFD2100902）
