食品工业科技2025，Vol.46Issue(9)：114-123,10.DOI:10.13386/j.issn1002-0306.2024050168
汉麻籽中靶向抑制胰脂肪酶/胆固醇酯酶多肽的快速鉴定筛选及活性探究
Insights Into Rapid Identification,Screening and Activity Investigation of Novel Hemp Seed Pancreatic Lipase/Cholesterol Esterase Inhibitory Peptides
摘要
Abstract
This study aimed to rapidly screen and identify pancreatic lipase(PL)and cholesterol esterase(CE)inhibitory peptides from hemp seed protein hydrolysates(HSPHs),and validate their activities,accompanied by preliminarily analyzing their molecular mechanisms.Initially,the PL and CE inhibitory activities of HSPHs were explored under different buffer systems.Subsequently,a combination of ultrafiltration,peptidomics,and virtual screening methods was employed for the rapid identification and screening of PL and CE inhibitory peptides.Finally,their activities were validated,and molecular docking was used to investigate the types of molecular interactions involved.The results indicated that,compared to conventional buffer systems,the simulated intestinal fluid system reduced the inhibitory effect of HSPHs on PL but enhanced their inhibitory effect on CE.Therefore,subsequent tests of PL and CE inhibitory activities were conducted using the simulated intestinal fluid system.The fractions of HSPHs smaller than 3 kDa,released by alcalase and papain,exhibited the highest PL/CE inhibitory activities.After identifying the peptide sequences,136 peptides with higher content and potential activity were selected to establish a candidate peptide library.From this library,14 peptides with lower binding energies were identified as PL and CE inhibitory peptides.In vitro activity validation demonstrated that APAM and RLPA exhibited significant inhibitory effects on both PL and CE.Molecular docking results suggested that APAM and RLPA might inhibit the activities of PL and CE by forming hydrogen bonds and hydrophobic interactions with the residues at the enzyme active sites and surface loops,thereby restricting the exposure of the active sites and the entry of substrates.This study indicates that hemp seeds are a potential source for developing lipid digestion enzyme inhibitory peptides,providing a reference for the subsequent development of lipid-lowering peptide products from hemp seeds.关键词
汉麻籽/生物活性肽/酶抑制/肽组学/虚拟筛选/分子对接Key words
hemp seed/bioactive peptide/enzyme inhibiting/peptidomics/virtual screening/molecular docking分类
轻工业引用本文复制引用
尹浩,朱将雄,钟宇,王丹凤,邓云,章敏燕,赵芳..汉麻籽中靶向抑制胰脂肪酶/胆固醇酯酶多肽的快速鉴定筛选及活性探究[J].食品工业科技,2025,46(9):114-123,10.基金项目
云南省基础研究计划项目(202301AS070014) （202301AS070014）
国家科技部十四五重点研发(子课题)(2023YFF1104302-1). （子课题）
