茶叶科学2025，Vol.45Issue(2)：253-265,13.
茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应
Identification of the L-type Lectin Receptor Kinase Gene Family in Camellia sinensis and Its Response to Tea Brown Blight and Tea Anthracnose
摘要
Abstract
L-type lectin receptor-like kinases(LecRKs)constitute a significant subfamily of plant receptor-like kinases,playing pivotal roles in plant development and immune responses.In this study,the CsLecRK gene family was systematically identified and analyzed in Camellia sinensis to explore their functions in growth,development and disease resistance.A total of 59 L-type CsLecRKs containing PF00069 and PF00139 domains were identified through HMMsearch analysis.Phylogenetic analysis shows these genes could be classified into 5 subgroups,revealing notable differences in exon-intron structures among the subgroups.Promoter region analysis shows enrichment of cis-acting elements associated with hormone responses and stress signaling pathways.Transcriptome data reveals that L-type CsLecRKs exhibited differential expressions in different tea plant tissues.qRT-PCR validation demonstrates that CsLecRK Ⅳ.1.2 and CsLecRK S.5 were significantly upregulated during the early stage of Colletotrichum camelliae infection and the later stages of Pestalotiopsis spp.infection.Notably,their expression levels were substantially higher in the resistant cultivar'Qianmei 419'compared to the susceptible cultivar'Qianmei 818',indicating their critical involvement in the immune response to pathogen infection.This study highlighted the critical mechanisms of L-type CsLecRKs in tea plant defenses against brown blight and anthracnose,elucidated the expression profiles of CsLecRK Ⅳ.1.2 and CsLecRK S.5 during pathogen infection,and demonstrated their potential to enhance disease resistance by activating the tea plant immune system.These findings provided valuable genetic resources and theoretical support for molecular breeding as well as green disease control strategies in tea plants.关键词
茶树/L型凝集素受体激酶/茶炭疽病/茶轮斑病/免疫反应Key words
tea plant/L-type lectin receptor kinases/tea anthracnose/tea brown blight/immune response分类
农业科学引用本文复制引用
李悦欣,鄢东海,张金峰,蒲运丹,李帅,孟泽洪..茶树L型凝集素受体激酶基因家族鉴定及其对茶轮斑病和茶炭疽病的响应[J].茶叶科学,2025,45(2):253-265,13.基金项目
贵州省科技计划项目(黔科合基础-ZK[2024]一般546)、贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023009)、黔农科博士基金[2025]06号 （黔科合基础-ZK[2024]一般546）
