茶叶科学2025，Vol.45Issue(2)：266-272,7.
茶树叶斑病病原菌的分离与鉴定
Isolation and Identification of the Pathogen Causing Leaf Spot in Tea Plants
摘要
Abstract
Tea plant[Camellia sinensis(L.)O.Kuntze]is an important agricultural economic crop in China.Tea leaf spot disease is one of the most important leaf diseases of tea plants.It seriously affects the yield and quality of tea.In 2023,a tea leaf spot disease was found in a tea garden in Menghai County,Xishuangbanna Dai Autonomous Prefecture Yunnan Province.In order to clarify the pathogen,the tissue isolation method and wound inoculation method were used to isolate and determine the pathogenicity of the pathogen.The morphological characteristics were observed and combined with molecular biology techniques to determine the type of pathogen.The results show that the three strains could infect tea leaves and were the causal agents of tea leaf spot.The pathogen causing tea leaf spot was identified as Nigrospora musae by observing its morphological characteristics and phylogenetic analysis of multiple genes(ITS,TUB2 and TEF-1α).The results of this study provided a theoretical basis for the prevention and control of tea leaf spot caused by the pathogen.关键词
茶树/叶斑病/黑孢菌/病原鉴定Key words
Camellia sinensis/leaf spot disease/Nigrospora musae/pathogen identification分类
农业科学引用本文复制引用
杨芳,江冰冰,雷金梅,郭存武,李丽梅,徐嘉忆,王兴华,袁文侠,王白娟..茶树叶斑病病原菌的分离与鉴定[J].茶叶科学,2025,45(2):266-272,7.基金项目
云南省乡村振兴科技专项(202304BI090013)、云南省重大科技专项计划(202302AE09002001)、云南省教育厅科学研究基金项目青年人才基础研究(2024J0406)、云南省建设面向南亚东南亚科技创新中心专项(202403AP140022) （202304BI090013）
