茶叶科学2025,Vol.45Issue(2):191-200,10.
茶树PATL基因家族鉴定及CsPATL1上游转录调控分析
Identification of CsPATL Gene Family and Analysis of Upstream Transcriptional Regulation of CsPATL1
摘要
Abstract
The Patellin(PATL)gene family is essential for plant growth,development and environmental adaptation.This study systematically identified and analyzed the CsPATL gene family in tea plants(Camellia sinensis).Five members of the CsPATL family were identified using a variety of bioinformatics techniques,and the physicochemical characteristics of their protein sequences were analyzed.The results show that the five CsPATL genes encode 232~585 amino acids,their molecular weights are 26.31~64.69 kDa,and their theoretical isoelectric points are 4.65~9.35.The chromosomal localization of these genes and the cis-elements in their promoter sequences were also analyzed in detail,and it was found that these genes were unevenly distributed on four chromosomes,and were mainly involved in phytohormone response,abiotic stress response,and light response,with light-responsive elements occupying a significant proportion in particular.Y1H,EMSA,and dual-luciferase assays confirm that Cshdz7 can directly bind to the CsPATL1 promoter and promote CsPATL1 gene expression.These findings provided a new perspective on the role of the CsPATL family genes in plant developmental regulation of tea plant development.关键词
茶树/基因家族/CsPATL1/启动子/转录调控Key words
Camellia sinensis/gene family/CsPATL1/promoter/transcriptional regulation分类
农业科学引用本文复制引用
王金波,谢思艺,窦祥亚,申小华,田娜,刘硕谦..茶树PATL基因家族鉴定及CsPATL1上游转录调控分析[J].茶叶科学,2025,45(2):191-200,10.基金项目
国家自然科学基金联合基金重点项目(U22A20500)、湖南省现代农业产业技术体系项目(HARS-10)、国家重点研发计划项目(2021YFD1200203)、国家茶树育种联合攻关项目(GJCSYZLHGG-12) (U22A20500)