茶叶科学2025，Vol.45Issue(2)：201-218,18.
'槠叶齐'叶绿体与线粒体基因组密码子偏好性分析
Analysis of Codon Usage Bias in Chloroplast and Mitochondrial Genomes of Camellia sinensis cv.'Zhuyeqi'
摘要
Abstract
Codon usage bias serves as an important driving force for gene expression regulation and molecular evolution,and is of particular importance in the study of plant organellar genomes.Camellia sinensis cv.'Zhuyeqi',an important tea cultivar in China,has not yet received a systematic report on the codon usage patterns of its organellar genomes.This study was systematic bioinformatic analysis of the 52 chloroplast-encoded genes and 29 mitochondrial-encoded genes of'Zhuyeqi'.The results reveal that:(1)both the chloroplast genome(ENC=44.64±3.25)and the mitochondrial genome(ENC=51.98±3.47)exhibit weak codon usage bias,with the chloroplast bias primarily driven by natural selection(GC3s and ENC correlation R2=0.482).While the mitochondrial bias is jointly influenced by natural selection and mutational pressure(R2=0.312).(2)Relative synonymous codon usage(RSCU)analysis demonstrates that both organellar genomes significantly prefer synonymous codons ending in A/U,and the highly expressed chloroplast genes(rpoC2,psbA)exhibit stronger codon preferences.(3)a multi-parameter screening approach identified 20 optimal chloroplast codons(GCA,GCU)and 23 optimal mitochondrial codons(GCC,AGG).This study provided elucidation of the codon usage characteristics and evolutionary driving forces in the organellar genomes of Camellia sinensis cv.'Zhuyeqi',offering crucial theoretical guidance for the optimization of the tea molecular breeding system and the efficient expression of exogenous genes.关键词
槠叶齐/叶绿体基因组/线粒体基因组/密码子使用偏好性/最优密码子Key words
Camellia sinensis cv.'Zhuyeqi'/chloroplast genome/mitochondrial genome/codon usage bias/optimal codons分类
植物保护学引用本文复制引用
曾文娟,朱友鹏,陈嘉欣,李洪玉,王双辉,龚意辉,陈致印..'槠叶齐'叶绿体与线粒体基因组密码子偏好性分析[J].茶叶科学,2025,45(2):201-218,18.基金项目
湖南省自然科学基金(2023JJ50465)、湖南省科技创新计划项目(2024RC8289)、娄底市科技创新计划项目(2023RC3501)、国家级大学生创新训练项目(S202310553022) （2023JJ50465）
