食品科学2025，Vol.46Issue(11)：1-10,10.DOI:10.7506/spkx1002-6630-20241116-128
硒化黄精多糖的制备、结构表征及体外抗氧化活性分析
Preparation,Structural Characterization and in Vitro Antioxidant Activity of Selenized Polygonatum sibiricum Polysaccharides
摘要
Abstract
Selenized Polygonatum sibiricum polysaccharides(PSP-Se)were prepared using a redox system containing glacial acetic acid and sodium selenite and characterized inductively coupled plasma atomic emission spectrometry(ICP-AES),gas chromatography-mass spectrometry(GC-MS),Fourier transform infrared(FTIR)spectroscopy,scanning electron microscopy(SEM),particle size and zeta potential analysis.The antioxidant activity of Polygonatum sibiricum polysaccharides(PSP)and PSP-Se were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical,2,2'-diazide-bis(3-ethyl-benzothiazole-6-sulfonic acid)(ABTS)cation radical assay and hydroxyl radical assays.The results showed that the optimal conditions for the preparation of PSP-Se were determined by response surface methodology(RSM)as follows:1.5 g of sodium selenite and 3.0 mL of glacial acetic acid per g of PSP,80℃and 12 h.The as-prepared PSP-Se contained(6.00±0.20)mg/g of Se,exhibited a spherical morphology with a particle size of 1 654 nm,and showed improved thermal stability and a 95.12%increase in the absolute value of the zeta potential compared with PSP.After selenium modification,the basic skeleton of PSP was preserved.Monosaccharide composition analysis showed that PSP-Se but not PSP contained N-acetyl-glucosamine and N-acetyl-galactosamine,with differences in the contents of shared monosaccharide components being observed between them.FTIR spectroscopy showed that two new absorption peaks appeared at 1 131 and 900 cm-1 in PSP-Se,indicating the formation of selenite ester bonds and successful selenization.In vitro antioxidant assays demonstrated that PSP-Se had significantly higher scavenging capacity against DPPH,ABTS cation and hydroxyl radical compared with PSP(P<0.05),in a dose-dependent manner.关键词
黄精多糖/硒化修饰/结构表征/抗氧化活性Key words
Polygonatum sibiricum polysaccharide/selenization modification/structural characterization/antioxidant activity分类
轻工业引用本文复制引用
赵新悦,王清玉,王柏璇,赵庆旭,李大鹏,杨悦..硒化黄精多糖的制备、结构表征及体外抗氧化活性分析[J].食品科学,2025,46(11):1-10,10.基金项目
国家自然科学基金青年科学基金项目(41907357) （41907357）
山东省重点研发项目(2021TZXD007) （2021TZXD007）
