食品科学2025，Vol.46Issue(11)：139-145,7.DOI:10.7506/spkx1002-6630-20240814-100
阴离子表面活性剂辛烯基琥珀酸淀粉钠对脂肪酶催化行为的影响
Effect of the Anionic Surfactant Sodium Starch Octenyl Succinate on the Catalytic Behavior of Lipase
摘要
Abstract
This study investigated the effect of the anionic surfactant sodium starch octenyl succinate(SSOS)on the catalytic characteristics of the lipase Novozym51032.The effect of their interaction on the structure of the enzyme was explored by ultraviolet(UV)spectroscopy,fluorescence spectroscopy,and Fourier transform infrared(FTIR)spectroscopy.The results showed that the presence of 6.0 mg/mL SSOS increased the relative activity of the lipase significantly by 164.99%.Kinetic analysis revealed that at a low mass concentration(1 mg/mL)of SSOS,the Michaelis constant(Km)of the lipase decreased from 4.12 to 3.46 μmol/L,but it increased to 58.82 μmol/L at a higher mass concentration(6 mg/mL),with the maximum reaction rate(Vmax)increasing to 333.00 μmol/(L·min).Fluorescence spectroscopy and FTIR spectroscopy showed that the fluorescence intensity of the lipase gradually weakened with increasing concentration of the surfactant,accompanied by secondary structure transformation of the enzyme from α-helix and β-turn to β-sheet.This suggested that the influence of SSOS on the catalytic characteristics of the lipase was associated with the enzyme's structural alteration.关键词
脂肪酶/阴离子表面活性剂/催化活性/动力学/光谱行为Key words
lipase/anionic surfactant/catalytic activity/kinetics/spectral behavior分类
生物科学引用本文复制引用
付薇薇,滕超,周明春,周亚迪,邱学宇,孙启杰..阴离子表面活性剂辛烯基琥珀酸淀粉钠对脂肪酶催化行为的影响[J].食品科学,2025,46(11):139-145,7.基金项目
北京市自然科学基金-市教委联合资助重点项目(KZ202010011018) （KZ202010011018）
