食品科学2025，Vol.46Issue(11)：208-216,9.DOI:10.7506/spkx1002-6630-20241115-122
肉苁蓉多糖的结构表征及对高糖诱导的肾小管上皮细胞损伤的保护作用
Structural Characterization of Cistanche deserticola Polysaccharides and Its Protective Effect on High Glucose-Induced HK-2 Cell Injury
摘要
Abstract
Objective:To investigate the structures of Cistanche deserticola polysaccharides(CDPs)and to evaluate the protective effect thereof on human renal proximal tubular epithelial(HK-2)cell injury and the underlying mechanism.Methods:The chemical composition,molecular mass,monosaccharide composition and possible structure of CDPs were detected using the sulfuric acid phenol method,the hydroxyl biphenyl method,the Coomassie brilliant blue method,ion chromatography,high performance gel permeation chromatography and infrared chromatography.The protective effect and mechanism of CDPs on high glucose-induced injury in HK-2 cells were evaluated.Results:The weight-average molecular masses of CDPs were 8 031,4 198,and 3 444 Da,which were primarily composed of glucose,galactose,rhamnose,mannose and arabinose,and may contain both α-and β-glycosidic bonds.CDPs reduced oxidative damage in HK-2 cells treated with high glucose and mitigated the impairment of mitochondrial membrane potential.The relative expression of Toll-like receptor 4(TLR4),phosphorylated nuclear factor(NF)-κB p65,and phosphorylated inhibitor α of NF-κB(p-IκBα)in HK-2 cells significantly decreased after intervention with different concentrations of CDPs(P<0.05).Conclusion:CDPs had the ability to reduce high glucose-induced renal tubular epithelial cell injury by inhibiting the TLR4/NF-κB signaling pathway to promote cell proliferation and inhibit oxidative stress and apoptosis.关键词
肉苁蓉多糖/肾小管上皮细胞/氧化应激/Toll样受体4/核因子-κB信号通路Key words
Cistanche deserticola polysaccharides/human renal tubular epithelial cells/oxidative stress/Toll-like receptor 4/nuclear factor-κB signaling pathway分类
医药卫生引用本文复制引用
罗欣杰,刘江云,杨建华,胡君萍..肉苁蓉多糖的结构表征及对高糖诱导的肾小管上皮细胞损伤的保护作用[J].食品科学,2025,46(11):208-216,9.基金项目
新疆维吾尔自治区自然科学基金重点项目(2021D01D11) （2021D01D11）
国家自然科学基金地区科学基金项目(82160772) （82160772）
新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713) （XJDX1713）
