中国食物与营养2025，Vol.31Issue(4)：29-33,40,6.
超高效液相色谱-大气压化学离子源-三重四极杆质谱联用技术同时测定海芦笋中的甾醇
Simultaneous Determination of Sterols in Sea Asparagus by UPLC-APCI-MS/MS
郑颜 1冯温泽 2蔡达 3冯立田 4赵善仓3
作者信息
- 1. 海南海渔生物科技有限公司,海口 570100||山东农业大学食品科学与工程学院,山东 泰安 271018||山东省农业科学院农业质量标准与检测技术研究所/山东省食品质量与安全检测技术重点实验室,济南 250100
- 2. 中国科学院海洋研究所,山东 青岛 266071
- 3. 山东省农业科学院农业质量标准与检测技术研究所/山东省食品质量与安全检测技术重点实验室,济南 250100
- 4. 海南海渔生物科技有限公司,海口 570100
- 折叠
摘要
Abstract
[Objective]To establish an ultra-performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry(UPLC-APCI-MS/MS)for the determination of stigmasterol,campesterol,β-sitosterol and stigmastanol by multi-reaction monitoring(MRM)mode in the sea asparagus.[Method]Using ACQUITY UPLC BEH C18 column(100 mm×2.1mm,1.7 µm),0.1%formic acid aqueous solution-methanol as the mobile phase,with a flow rate of 0.5 mL/min.Using atmospheric pressure chemical ion source(APCI),and selected multi-reaction ion monitoring(MRM)detection mode.[Result]Totally 4 sterols had good linear relationships in the linear ranges of 2-1 000 µg/kg and 5-1 000 µg/kg,and the recoveries were between 92%—112%.The LODs of stigmasterol,campesterol,β-sitosterol,stigmastanol were 5 µg/kg,2 µg/kg,2 µg/kg,5 µg/kg,and their LOQs were 10 µg/kg,5 µg/kg,5 µg/kg,10 µg/kg respectively.[Conclusion]Due to its high sensitivity and fast analysis speed,this method has been successfully applied to the analysis of sterol content in different harvest stages of sea asparagus,which provides a reference for the determination of other phytosterol content.关键词
海芦笋/UPLC-APCI-MS/MS/甾醇Key words
sea asparagus/UPLC-APCI-MS/MS/sterols引用本文复制引用
郑颜,冯温泽,蔡达,冯立田,赵善仓..超高效液相色谱-大气压化学离子源-三重四极杆质谱联用技术同时测定海芦笋中的甾醇[J].中国食物与营养,2025,31(4):29-33,40,6.
