中外葡萄与葡萄酒Issue(3)：79-86,8.DOI:10.13414/j.cnki.zwpp.2025.03.009
基于代谢组学的'黑皇'葡萄双季果挥发性差异代谢物分析
Analysis of Volatile Differential Metabolites of'Black Emperor'Grapes Dual-cropping Fruits Based on Broadly Targeted Metabolomics
摘要
Abstract
To investigate the differences of volatile aroma metabolites between summer grape fruits(SF)and winter grape fruits(WF)under a two-crop-a-year cultivation system,this study utilized headspace solid phase microextraction(HS-SPME)and gas chromatography-mass spectrometery(GC-MC)to analyze the volatile aroma metabolites of'Black Emperor'grapes using selected ion monitoring(SIM)mode.A total of 602 volatile compounds were detected in the summer and winter grape fruits,primarily consisting of terpenes,followed by esters,heterocyclic compounds and others.The contents of terpenes,ketones and hydrocarbons between WF and SF were significantly different,with 21 significantly different metabolites,including 10 up-regulated metabolites and 11 down-regulated metabolites.SF accumulates higher concentrations of terpene substances than WF,indicating that the metabolism of terpene substances in'Black Emperor'grapes responds to climate conditions in a variety-specific manner.Flavoromics analysis showed that fruit aroma and sweetness were the most critical aroma and taste of'Black Emperor'grapes,the relevant substances were(Z)-3-hexenal,benzaldehyde,citronellal,1-(2,6,6-trimethyl-1-cyclohexen-1-yl)-2-buten-1-one and 1-(4-methoxyphenyl)-2-propanone.This study enriches the new theory of volatile aroma metabolism in two-crop grapes,and provides reference basis for selecting research grapes with suitable aroma for two-crop-a-year cultivation.关键词
'黑皇'葡萄/一年两收栽培/代谢组学分析/香气成分Key words
'Black Emperor'grape/two-crops-a-year cultivation/metabolomic analysis/aroma components分类
园艺学与植物营养学引用本文复制引用
黄桂媛,郭荣荣,程昌富,时晓芳,盘丰平,韩佳宇,林玲,张瑛..基于代谢组学的'黑皇'葡萄双季果挥发性差异代谢物分析[J].中外葡萄与葡萄酒,2025,(3):79-86,8.基金项目
财政部和农业农村部:国家现代农业产业技术体系项目(CARS-29-21)广西现代农业产业技术体系葡萄创新团队(nycytxgxcxtd-20-01)广西壮族自治区农业科学院基本科研业务专项项目(桂农科2024YP101) （CARS-29-21）
