食品与发酵工业2025，Vol.51Issue(10)：1-8,8.DOI:10.13995/j.cnki.11-1802/ts.039668
代谢改造谷氨酸棒杆菌高效发酵生产L-高丝氨酸
Metabolic engineering of Corynebacterium glutamicum for high-level fermentative production of L-homoserine
摘要
Abstract
L-homoserine is a non-protein chiral amino acid with broad application potential in the fields of food,feed,medicine,and agriculture.However,there are still limited examples of exploring the GRAS(generally regarded as safe)Corynebacterium glutamicum for fermentative production of L-homoserine.This study employed the wild-type C.glutamicum ATCC 13032 as an initial strain for designing and constructing L-homoserine producer.Firstly,this study attenuated the L-homoserine competitive pathway by knocking out thrB,alaT,and ddh,and overexpressed lysC,hom,and ppc to enhance L-homoserine biosynthetic flux,thus achieving effective L-homoserine accumulation.Subsequently,this study further enhanced L-homoserine production by improving intracellular NADPH supply and enhancing L-homoserine efflux capacity.Finally,through engineering and reshaping the glucose uptake and utilization system,an efficient biosynthesis of L-homo-serine was achieved in C.glutamicum.The optimal engineered strain could accumulate 9.29 g/L L-homoserine in shake flasks and 77.53 g/L L-homoserine in a 5-L fermenter with a production rate of 1.29 g/L/h,representing the highest reported level of L-homoserine production by C.glutamicum.This study will provide a valuable reference for the construction of high-producing L-homoserine strains.关键词
L-高丝氨酸/谷氨酸棒杆菌/代谢工程/细胞工厂/发酵Key words
L-homoserine/Corynebacterium glutamicum/metabolic engineering/microbial cell factory/fermentation引用本文复制引用
钟兆月,马振平,祁玉婷,张霞,徐宁,刘君,邵丽,姜巨全..代谢改造谷氨酸棒杆菌高效发酵生产L-高丝氨酸[J].食品与发酵工业,2025,51(10):1-8,8.基金项目
国家自然科学基金联合基金重点项目(U23A20143) （U23A20143）
天津市合成生物技术创新能力提升行动项目(TSBICIP-KJGG-010) （TSBICIP-KJGG-010）
