食品与发酵工业2025，Vol.51Issue(10)：23-32,10.DOI:10.13995/j.cnki.11-1802/ts.039915
抑制单增李斯特菌的益生乳酸菌筛选及原位抑制效果研究
Screening and in situ inhibitory effects of probiotic lactic acid bacteria against Listeria monocytogenes
摘要
Abstract
Dairy products are susceptible to contamination by Listeria monocytogenes,leading to serious health concerns.This study aimed to screen lactic acid bacteria with potent inhibitory activity against L.monocytogenes and evaluate their probiotic properties,safety,and in situ inhibitory effects in pasteurized milk.Twenty-six lactic acid bacteria strains were isolated from 9 yoghurt lump samples from Xinjiang,China.Among them,5 strains exhibited significant inhibitory activity against L.monocytogenes as determined by the agar well diffusion method and characterized as Limosilactobacillus reuteri XJ-4,Pediococcus acidilactici XJ-14,Pediococcus acidilactici XJ-7,Pedi-ococcus acidilactici XJ-24,and Limosilactobacillus reuteri XJ-47 based on morphological and 16S rDNA analyses.Evaluation of probiotic potential revealed that L.reuteri XJ-47 exhibited the highest tolerance to simulated gastrointestinal fluids.All 5 strains displayed excellent bile salt tolerance,auto-aggregation,and co-aggregation capabilities,with P.acidilactici XJ-14 and P.acidilactici XJ-7 demonstrating the highest hydrophobicity.Safety assessments indicated that all 5 strains were sensitive to penicillin,ceftriaxone,erythromycin,and chloram-phenicol,with no hemolytic activity.In pasteurized milk,P.acidilactici XJ-24 exhibited the strongest inhibition of L.monocytogenes,re-ducing its count by 1.71 lg CFU/mL.Additionally,P.acidilactici XJ-24 almost completely suppressed L.monocytogenes in pre-fermented pasteurized milk.Scanning electron microscopy revealed that P.acidilactici XJ-24 could adhere to L.monocytogenes and significantly dis-rupt its cellular morphology.This study provides a theoretical foundation for the application of these 5 lactic acid bacteria strains in the fields of probiotics and the dairy industry.关键词
单增李斯特菌/乳酸菌/益生潜力/原位抑制/巴氏灭菌乳Key words
Listeria monocytogenes/lactic acid bacteria/probiotic potential/in situ inhibition/pasteurized milk引用本文复制引用
胡伟中,李慧颖,史早,杨雪,易志强,周姝芯,阚建全,杜木英..抑制单增李斯特菌的益生乳酸菌筛选及原位抑制效果研究[J].食品与发酵工业,2025,51(10):23-32,10.基金项目
科技部国家重点研发计划项目(2022YFE0120800) （2022YFE0120800）
