食品与发酵工业2025，Vol.51Issue(10)：33-38,6.DOI:10.13995/j.cnki.11-1802/ts.039598
甘油葡糖苷的全细胞固定化酶法制备及应用评价
Enzymatic preparation of 2-O-α-D-glucopyranosyl-sn-glycerol by whole-cell immobilization and evaluation of its application
摘要
Abstract
2-O-α-D-Glucopyranosyl-sn-glycerol(2-αGG)has high moisturizing and antioxidant properties,making it a promising in-gredient in cosmetics and pharmaceuticals.In this study,the production of 2-αGG was achieved by immobilizing cells that produced su-crose phosphorylase.The optimal conditions for the immobilization of cells using κ-carrageenan were determined.Immobilized cell pellets were prepared by combining 0.02 g/mL κ-carrageenan with cells at a concentration of 0.12 mg/mL.At a glycerol-sucrose concentration of 1 mol/L,a 39 g/L yield of 2-αGG was obtained after a 48-hour reaction at 35 ℃.Moreover,the impact of 2-α GG on the survivability of HepG2 cells was studied.The higher the concentration,the greater the inhibitory effect observed on HepG2 cells.After treating HepG2 cells induced by free fatty acids(FFA)with 2-αGG,comparing the cells with the model group,the experimental group showed reduced intracellular lipid droplets,decreased levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),alanine aminotransferase(ALT),and aspartate aminotransferase(AST).2-αGG has the potential to improve lipid deposition induced by FFA in non-alcoholic fatty liver cell.关键词
2-α甘油葡萄糖苷/细胞固定化/κ-卡拉胶/非酒精性脂肪肝病Key words
2-O-α-D-glucopyranosyl-sn-glycerol(2-αGG)/cell immobilization/κ-carrageenan/non-alcoholic fatty liver disease引用本文复制引用
左玉洁,孙孟筱,孙晶晶,王伟,江承程,郝建华..甘油葡糖苷的全细胞固定化酶法制备及应用评价[J].食品与发酵工业,2025,51(10):33-38,6.基金项目
国家重点研究发展计划项目(2022YFC2805101) （2022YFC2805101）
青岛海洋科技中心项目(2022QNLM030003-2) （2022QNLM030003-2）
青岛市自然科学基金项目(23-2-1-185-zyyd-jch) （23-2-1-185-zyyd-jch）
中国水产科学研究院基本科研业务费资助项目(2023TD71) （2023TD71）
