食品与发酵工业2025，Vol.51Issue(10)：56-62,7.DOI:10.13995/j.cnki.11-1802/ts.038782
细菌素高产菌株的诱变选育及其液体发酵动力学分析
Mutation breeding and its liquid fermentation kinetics analysis of bacteriocin high-yielding strains
摘要
Abstract
This study aimed to increase the content of the novel bacteriocin Plantarum C010 produced by Lactobacillus plantarum C010.L.plantarum C010 was mutagenized by ultraviolet radiation,nitrite,acridine orange,and laser,and double plate antagonistic inhibition zone method and high-performance liquid chromatography were used to screen out the high-yielding mutant strains.Results showed that all four mutagenesis treatments could improve the production of bacteriocin Plantarum C010.The mutant strain JG-2 that was mutagenized and screened out by laser reached 17.79 mg/mL on bacteriocin Plantarum C010 production,which was 85.31％ higher than the original strain C010(9.60 mg/mL).Furthermore,the kinetic analysis of liquid batch fermentation showed that the model fitting determination coefficients(R2)of cell growth,total sugar consumption,and product synthesis of strains C010 and JG-2 were better than others.Bacteriocin synthesis of the two strains was partially cell growth-coupled,and the bacteriocin synthesis parameters of the mutant strain JG-2 were better than other original strain C010.The results provide data basis and research ideas to improve bacteriocin Plantarum C010 production.关键词
植物乳杆菌C010/细菌素/诱变选育/高产突变株/动力学分析Key words
Lactobacillus plantarum C010/bacteriocin/mutation breeding/high-yielding strain/kinetics analysis引用本文复制引用
李梦瑶,徐静宇,肖四生,杨代康,徐靖宇,姚明印,黄林..细菌素高产菌株的诱变选育及其液体发酵动力学分析[J].食品与发酵工业,2025,51(10):56-62,7.基金项目
国家自然科学基金地区科学基金项目(31560482) （31560482）
江西农业大学大学生创新创业训练计划项目(202210410257) （202210410257）
