食品与发酵工业2025，Vol.51Issue(10)：77-87,11.DOI:10.13995/j.cnki.11-1802/ts.040344
脱镁叶绿酸a缓解高脂膳食诱导的小鼠肝肠损伤研究
Studies on alleviation of high-fat diet-induced liver and intestinal damage by pheophorbide a
摘要
Abstract
This paper combined histopathology,blood biochemistry,and high-throughput analytical techniques(microbial diversity and metabolomics of liver and flora)to study the mitigating effect and mechanism of different concentrations(0.16,0.12,0.08,0.04 mg/mL)of pheophorbide a(Pheo a)on the liver and intestinal damage induced by high-fat diet.Body weight results showed that the body weight of mice in the high-concentration group(0.16,0.12 mg/mL)was significantly lower than that of the high-fat dietary control group(P＜0.05).Blood glucose values were measured and found to be in the normal range in the high-concentration group,while the low-concentra-tion group(0.08,0.04 mg/mL)and the high-fat dietary control group were in the range of pre-diabetic blood glucose values.The results of serum indexes showed that pheophorbide a significantly reduced the high-fat diet-induced triglyceride,insulin,IL-6,IL-1β,and ma-londialdehyde expression levels(P＜0.05)in a dose-dependent manner.Liver metabolomics results showed significant changes in metabo-lites of nicotinoylglycine,succinic acid,alanine,glutamic acid,and valine(P＜0.05).Microbial diversity results revealed that the ex-perimental group significantly increased the abundance of Muribaculaceae and Lactobacillus and decreased the abundance of Clostridium at the genus level(P＜0.05).Metabolomics of intestinal flora revealed significant changed in metabolites such as oxalacetic acid,methyl-2-hydroxyisobutyric acid,and α-ketoglutaric acid(P＜0.05).In conclusion,pheophorbide a could effectively delay weight gain and have a regulatory effect on the intestinal flora of mice,significantly reversing high-fat diet-induced intestinal flora dysbiosis and reducing high-fat diet-induced low-grade inflammation,which in turn alleviates the metabolic syndrome and promotes the health of the organism,and the corresponding regulatory mechanisms need to be studied in depth.关键词
脱镁叶绿酸a/高脂膳食/肝脏损伤/肠道菌群/代谢综合征Key words
pheophorbide a/high-fat diet/liver damage/gut flora/metabolic syndrome引用本文复制引用
刘若曦,曾全恒,蔡甜,陈科伟..脱镁叶绿酸a缓解高脂膳食诱导的小鼠肝肠损伤研究[J].食品与发酵工业,2025,51(10):77-87,11.基金项目
国家重点研发计划(2023YFE0116100) （2023YFE0116100）
重庆市科委基础研究与前沿探索项目(自然科学基金CSTB2022NSCQ-MSX0633) （自然科学基金CSTB2022NSCQ-MSX0633）
西南大学创新研究2035先导计划重点项目(SWU-XDZD22007) （SWU-XDZD22007）
