食品与发酵工业2025，Vol.51Issue(10)：224-232,9.DOI:10.13995/j.cnki.11-1802/ts.039838
矢车菊素-3-O-葡萄糖苷和矢车菊素对酪氨酸酶的抑制作用及其机理研究
Inhibition effects and mechanisms of cyanidin-3-O-glicoside and cyanidin on tyrosinase
摘要
Abstract
Tyrosinase is the main rate-limiting enzyme of melanogenesis,and inhibition of tyrosinase activity is one of the effective ways to reduce melanogenesis and thus achieve a whitening effect.In this experiment,the inhibitory effects and mechanisms of cyanidin-3-O-glucoside(C3G)and cyanidin(Cy)on tyrosinase were comprehensively investigated using enzyme inhibition kinetic analysis,ultravio-let spectroscopy,infrared spectroscopy,fluorescence spectroscopy,circular dichroism spectroscopy,and molecular docking simulation a-nalysis while using mushroom tyrosinase as the target.Results showed that the inhibitory activity of C3G(IC50=124.8 μmol/L)and Cy(IC50=278.1 μmol/L)on tyrosinase were better than that of the positive control kojic acid(IC50=536.4 μmol/L).The inhibitory effect of C3G and Cy on tyrosinase was reversible,and the inhibitory effect of C3G on tyrosinase was competitive inhibition,while the inhibitory effect of Cy on tyrosinase was mixed inhibition.Results of ultraviolet,Fourier transform infrared spectroscopy,and circular dichroism spec-tra showed that the binding of C3G and Cy to tyrosinase resulted in the conformation change of tyrosinase,which led to the inhibition of the enzyme activity.Fluorescence quenching results showed that C3G and Cy combined with tyrosinase changed the microenvironment of Tyr and Trp residues,resulting in static quenching.Synchronous fluorescence results showed that the combination of C3G and Cy with tyrosi-nase enhanced the microenvironmental polarity of Tyr and Trp residues and reduced the hydrophobic ability.Three-dimensional fluores-cence spectra showed that both C3G and Cy could reduce the intensity of spectral characteristic peaks of tyrosinase.Results of the multi-spectral analysis showed that the inhibitory effect of C3G on tyrosinase was stronger than that of Cy.Molecular docking simulation results showed that C3G formed six hydrogen bonds with amino acid residues HIS178,ASN174,GLN41,and GLN44 of tyrosinase,and π-π in-teraction was generated with amino acid residues GLU173 and LYS180.Cy bound to tyrosinase and formed four hydrogen bonds with amino acid residues ASN174,GLU173,GLN41,and GLN44,resulting in π-π interaction with amino acid residues GLU173.Molecular docking results further demonstrated that C3G bound tightly to residues in the active cavity of tyrosinase and had a higher affinity for tyrosinase compared to Cy.The conclusions of the inhibitory enzyme activity experiment and multispectral experiment were further supplemented.This study provides a reference for further research and development of polyphenols such as C3G and Cy in the field of tyrosinase inhibitors and acts as scientific support for the development of natural high-efficiency tyrosine inhibitors for cosmetic whitening additives.关键词
酪氨酸酶/矢车菊素-3-O-葡萄糖苷/矢车菊素/光谱/分子对接Key words
tyrosinase/cyanidin-3-O-glicoside/cyanidin/spectrum/molecular docking引用本文复制引用
张春子,马小燕,阿布里孜·阿不都热合曼,庞土霞,马晓丽..矢车菊素-3-O-葡萄糖苷和矢车菊素对酪氨酸酶的抑制作用及其机理研究[J].食品与发酵工业,2025,51(10):224-232,9.基金项目
自治区天山英才青年拔尖人才项目(2022TSYCCX0104) （2022TSYCCX0104）
