食品工业科技2025，Vol.46Issue(11)：124-131,8.DOI:10.13386/j.issn1002-0306.2024120147
不同金属盐离子对乳清分离蛋白纤维聚合物形成的影响
Effects of Different Metal Ions on the Formation of Fibril Aggregates of Whey Protein Isolate
摘要
Abstract
In this study,the effects of different metal ions(NaCl,KCl,CaCl2,AlCl3,and FeCl3)on the polymerization kinetics,morphology and structure of the fibril aggregates formed from whey protein isolate(WPI)were investigated.The results indicated that the final structure and characteristics of WPI fibril aggregates were affected by the different metal ions.NaCl,KCl,and CaCl2 exhibited minimal influence on the aggregation behavior of whey protein isolate(WPI).The pH and the conductivity of the final WPI fibril aggregate solutions were increased by NaCl,KCl,and CaCl2.While the aggregation of WPI was accelerated,the pH of the solution was reduced,and the conductivity of the solution was increased by AlCl3 and FeCl3.Compared with the thioflavin T(Th T)fluorescence intensity(388.92)of the native WPI fibril aggregates,the Th T fluorescence intensity increased to 465.39,433.37,and 486.83 in response to the addition of NaCl,KCl,and CaCl2,respectively,indicating an increase in the production of WPI fibril aggregates.In contrast,the Th T fluorescence intensity was reduced to 228.81 and 90.24 following the addition of AlCl3 and FeCl3,respectively,thereby indicating a reduction in the amounts of fibril aggregates generated.In addition,different metal ions altered the polymerization kinetics of WPI,reduced the(df/dt)max value,and shortened or prolonged the period of hysteresis.The presence of AlCl3 and FeCl3 was found to be conducive to the clustering of WPI fibril aggregates,which became coarser and more disordered.Moreover,the Fourier transform infrared spectroscopy results indicated that NaCl,KCl,and CaCl2 promoted the formation of β-sheet structures,whereas AlCl3 and FeCl3 inhibited the formation of these structures,and it was also found that addition of different metal ions altered the characteristic distances between β-chains within the fibril aggregates.These findings will provide a scientific basis for the preparation of fibril aggregates characterized by different aggregation forms using WPI.关键词
乳清分离蛋白/聚合动力学/金属离子/纤维聚合物/电导率/硫黄素TKey words
whey protein isolate/polymerization kinetics/metal ions/fibril aggregates/conductivity/thioflavin T分类
轻工纺织引用本文复制引用
董世鹏,刘美艳,邹霈怡,刘丹,陈鑫,李然红..不同金属盐离子对乳清分离蛋白纤维聚合物形成的影响[J].食品工业科技,2025,46(11):124-131,8.基金项目
黑龙江省教育厅基本科研项目(1452MSYYB005 （）
1453ZD024). （）
