食品工业科技2025，Vol.46Issue(11)：142-150,9.DOI:10.13386/j.issn1002-0306.2024060437
北风类芽孢杆菌木葡聚糖酶的表达、性质及在苹果渣木葡寡糖制备中的应用
Expression and Characterization of Xyloglucanase from Paenibacillus borealis and Its Application in the Production of Xylogluco-oligosaccharides from Apple Pomace
摘要
Abstract
Apple pomace(AP)is rich in xyloglucan,serves as a substrate to produce xylogluco-oligosaccharides with various functional activities.The hydrolysis of AP by novel xyloglucanases is conducive to the high-value utilization of AP.In this study,a novel GH74 xyloglucanase gene(PbXEG74B)from Paenibacillus borealis was cloned and expressed in Escherichia coli BL21(DE3).The enzyme was characterized and further used to hydrolyze AP for xylogluco-oligosaccharides production.It was expressed in E.coli with an expression level of 18.0 U/mL.The optimal conditions of PbXEG74B were pH5.5 and 55 ℃.It showed good stability in the pH range of 4.5～8.5 and temperatures up to 40℃.The enzyme hydrolyzed xyloglucan to produce xylogluco-oligosaccharides with degrees of polymerization(DP)of 2～9.AP was pretreated with deep eutectic solvents(DESs)and hydrolyzed by PbXEG74B to produce xylogluco-oligosaccharides.Under optimal conditions,the yield of xylogluco-oligosaccharides with DP 2～9 was 2.79 g/100 g AP,containing 67.0％,11.5％,and 21.5％of xylogluco-oligosaccharides with DP 2～4,5～6,and 7～9,respectively.PbXEG74B with good enzymatic and hydrolytic properties shows great potential in the high value utilization of AP.关键词
北风类芽孢杆菌/木葡聚糖酶/苹果渣/木葡寡糖/低共熔溶剂Key words
Paenibacillus borealis/xyloglucanase/apple pomace/xylogluco-oligosaccharide/deep eutectic solvents分类
生物科学引用本文复制引用
田嘉欣,杨行,赵玉琪,闫巧娟,李延啸,江正强..北风类芽孢杆菌木葡聚糖酶的表达、性质及在苹果渣木葡寡糖制备中的应用[J].食品工业科技,2025,46(11):142-150,9.基金项目
国家自然科学基金(22378419). （22378419）
