食品工业科技2025，Vol.46Issue(11)：213-223,11.DOI:10.13386/j.issn1002-0306.2024060232
葵花籽蛋白美拉德肽的制备及其呈味研究
Preparation and Flavor Characteristics of Maillard Peptides from Sunflower Seed Protein
摘要
Abstract
In order to prepare the Maillard peptides of the sunflower seed protein with higher umami,the enzymatic hydrolysis conditions of sunflower seed protein were optimized using the sunflower seed protein as raw material,and electronic tongue,sensory score,soluble nitrogen and hydrolysis degree as indicators,while the single factor experiment and orthogonal experiment were used to optimize the process conditions for preparing the umami peptides of sunflower seed protein using brown degree,color difference,amino nitrogen,electronic tongue and sensory score as indicators.The results showed that the electronic tongue score and sensory score of hydrolysate prepared was high using alkaline protease and flavor protease step-by-step enzymolysis,at 8％of substrate concentration,5000 U/g of the amount of enzyme addition,and 1∶2 of alkaline protease and flavor protease(enzyme activity ratio),with the enzymolysis of alkaline protease at 40 ℃for 3 h,and the enzymolysis of flavor enzyme at 50 ℃ for 1 h.The prepared umami peptides of sunflower seed protein had the highest electronic tongue score and umami amino acid content with 1.0％of xylose addition,initial pH7.0,at the Maillard reaction of 120 ℃ for 60 min.The molecular weight distribution in the umami peptides of sunflower seed protein before and after Maillard reaction was measured,which found that 500～1000 Da might be the main active peptides segment in the Maillard reaction.关键词
葵花籽蛋白/酶解/美拉德反应/鲜味肽Key words
sunflower seed protein/enzymolysis/Maillard reaction/umami peptide分类
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刘秀梅,王媛,李梦雨,张玉玉,孙宝国,张思雨,陈鸿剑,张艳荣,杨雅利..葵花籽蛋白美拉德肽的制备及其呈味研究[J].食品工业科技,2025,46(11):213-223,11.基金项目
延安苹果产业中关键问题技术培训,陕西省科技厅,一般项目-乡村振兴科技专项(2023-XCZX-09) （2023-XCZX-09）
基于赖氨酸二肽和葡萄糖的Maillard模型形成浓香葵花籽油中吡嗪类风味化合物的机理研究,国家自然科学基金青年科学基金项目(31701563) （31701563）
科技成果数字画像构建关键技术与应用研究,中国标准化研究院院长基金项目(242023Y-10413) （242023Y-10413）
国家市场监督管理总局科技计划项目(2023MK195). （2023MK195）
