食品工业科技2025，Vol.46Issue(11)：338-345,8.DOI:10.13386/j.issn1002-0306.2024070078
野菊花黄酮提取物对秀丽隐杆线虫氧化应激抗性作用研究
Chrysanthemum indicum Flavonoid Extract on the Oxidative Stress Resistance of Caenorhabditis elegans
摘要
Abstract
To investigate the in vivo antioxidant activity of flavonoid extract from Chrysanthemum indicum and its effects on oxidative stress resistance,this study evaluated the effects of Chrysanthemum indicum flavonoid extract on the antioxidant enzyme activities and the mean lifespan of Caenorhabditis elegans(C.elegans)under oxidative stress.Moreover,the mechanisms of enhancing oxidative stress resistance in nematodes were explored by utilizing a variety of transgenic strains.The results showed that pretreatment with Chrysanthemum indicum flavonoid extract significantly prolonged the mean lifespan of worms under oxidative stress by 14.00％,27.15％,and 18.65％(P＜0.01)at concentrations of 50,200,and 300 μg/mL,respectively.In addition,Chrysanthemum indicum flavonoid extract significantly increased the SOD activity and GSH level of C.elegans(P＜0.01).Furthermore,the nuclear proportion of SKN-1 was increased from 17.16％to 51.46％in LD1 strains when treated with 200 μg/mL Chrysanthemum indicum flavonoid extract.Additionally,the results of lifespan assay of EU1 strains indicated that the lifespan extension effect of Chrysanthemum indicum flavonoid extract under oxidative stress was relied on skn-1.The mechanism of enhancing oxidative stress resistance of Chrysanthemum indicum flavonoid extract might occur by activating the transcription factor SKN-1,which in turn increased the expression of antioxidant-related proteins SOD-3,GCS-1 and GST-4.This study provides a scientific basis for the application of Chrysanthemum indicum flavonoids in alleviating oxidative stress damage.关键词
野菊花黄酮提取物/秀丽隐杆线虫/抗氧化/氧化应激抗性Key words
Chrysanthemum indicum flavonoid extract/Caenorhabditis elegans/antioxidant/oxidative stress resistance分类
轻工纺织引用本文复制引用
崔海林,唐海轮,刘恋,李佐昊,向晶晶,李蓉..野菊花黄酮提取物对秀丽隐杆线虫氧化应激抗性作用研究[J].食品工业科技,2025,46(11):338-345,8.基金项目
湖北省教育厅科学技术研究项目(Q20234303) （Q20234303）
荆楚理工学院大学生创新创业训练计划项目(S202411336029) （S202411336029）
荆楚理工学院博士启动金项目(YY202403). （YY202403）
