乳业科学与技术2025，Vol.48Issue(3)：55-62,8.DOI:10.7506/rykxyjs1671-5187-20241217-110
分离自生牛乳的一株高产蛋白酶蜡样芽孢杆菌特性
Characteristics of a Protease-Producing Strain of Bacillus cereus Isolated from Raw Milk
摘要
Abstract
Eight Bacillus cereus strains were isolated from 10 batches of raw milk and identified through 16S rDNA sequencing and matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS).Out of these strains,one was selected on the basis of higher protease production and identified as B.cereus LY-6.We inoculated this strain back into skim milk and observed the resulting spoilage process.It was found that the milk turned to yellow,and had a bitter taste and an unpleasant odor.Moreover,the pH decreased and large amounts of proteases were found,indicating that the spoilage process is closely related to microbial protease production.Strain LY-6 was intolerant to ultra-high temperature sterilization.A distribution map of B.cereus in the dairy was drawn to control its spread.It was found that this bacterium mainly existed in places where milk stains are difficult to clean such as pasture bedding,towels,milk trucks,workshop floors,personnel shoe soles,and equipment surfaces.Therefore,special attention should be paid to the cleaning effect of the backend pipeline after the sterilization process to prevent potential food safety hazards caused by secondary contamination of B.cereus.关键词
蜡样芽孢杆菌/生牛乳/蛋白酶/苦味/16S rDNA/基质辅助激光解吸电离飞行时间质谱Key words
Bacillus cereus/raw milk/protease/bitterness/16S rDNA/matrix-assisted laser desorption ionization time-of-flight mass spectrometry分类
轻工纺织引用本文复制引用
屈雅莉,张彦辉,夏兆田,陈克良,周婷,楚珍珍,李飞..分离自生牛乳的一株高产蛋白酶蜡样芽孢杆菌特性[J].乳业科学与技术,2025,48(3):55-62,8.基金项目
河北省创新能力提升计划项目(24462802D) （24462802D）
