茶叶科学2025，Vol.45Issue(3)：427-438,12.
一株灰茶尺蠖高毒力金龟子绿僵菌的筛选、鉴定与培养条件
Screening,Identification and Culture Conditions of a Highly Virulent Metarhizium anisopliae Strain against Ectropis grisescens Warre
摘要
Abstract
As an important entomogenous fungus,Metarhizium has been extensively utilized in the biological control of agricultural and forestry pests.This study aimed to screen the most infective strain against E.grisescens from 7 preserved strains and verify its infectivity at different doses.Subsequently,the taxonomic status of the strain was determined through observation of morphological characteristics and methods for multigene phylogenetic tree analysis.Finally,the optimal culture medium,temperature and pH value for strain growth were investigated.The results indicate that the seven tested strains exhibited different control effects on the 3rd instar larvae of E.grisescens,with strain Ma54 exhibiting the highest virulence,achieving a corrected mortality rate of 81.67%after 10 d of treatment and an LT50(lethal time for 50%mortality)of 4.93 d.The mortality rate of E.grisescens shows a dose-response relationship with the spore concentration of strain Ma54.After 7 d of treatment under a high concentration of Metarhizium(1.0×108 spores·mL-1),the mortality rate reached 98.33%,and the LT50 value was 4.09 days.Seven days after strain Ma54 infected E.grisescens,the cadavers were covered with mycelia and turned dark green.After 14 days of growth on PDA medium,the conidia further spread and appeared dark green.The phylogenetic trees of the ribosomal transcription factor,β-tubulin,and the largest subunit of RNA polymerase Ⅱ of strain Ma54 all clustered with Metarhizium anisopliae.The colony diameter,spore production per unit area,and spore germination rate of strain Ma54 were optimal under conditions of a PPDA medium,a temperature of 25℃,and a pH value of 7.0.This study obtained the Ma54 strain with high virulence against E.grisescens,authenticated it as M.anisopliae,and preliminarily clarified its growth conditions of the strain,laying a foundation for its subsequent utilization in the biological control of E.grisescens.关键词
灰茶尺蠖/金龟子绿僵菌/高毒力/分类地位/生长条件Key words
Ectropis grisescens/Metarhizium anisopliae/high virulence/taxonomic status/growth conditions分类
农业科学引用本文复制引用
李良德,李金玉,李慧玲,张辉,王定锋..一株灰茶尺蠖高毒力金龟子绿僵菌的筛选、鉴定与培养条件[J].茶叶科学,2025,45(3):427-438,12.基金项目
福建省属公益类科研院所基本科研专项(2021R1029009)、福建省农业科学院青年英才项目(YC2021008)、福建省农业科学院科技创新团队项目(CXTD2021001-3) （2021R1029009）
