茶叶科学2025，Vol.45Issue(3)：439-453,15.
小贯小绿叶蝉卵黄原蛋白基因(EonuVg)克隆和功能研究及其对吡虫啉的响应
Cloning and Functional Analysis of Vitellogenin Genes(EonuVg)in Empoasca onukii and Their Response to Imidacloprid
摘要
Abstract
This study systematically investigated the molecular characteristics,expression patterns of the vitellogenin(Vg)gene EonuVg,and its response to imidacloprid in Empoasca onukii.The full-length cDNA sequences of two EonuVg genes,EonuVg1(PP128347)and EonuVg2(PP128348)were cloned and designated.Their open reading frames(ORFs)are 6 066 bp and 6 138 bp,encoding 2 021 and 2 045 amino acids,respectively.Both proteins contain conserved structural domains of Vg,including a signal peptide,Vitellogenin-N superfamily(Vit-N),Vit_open_b-sht(Vit-b)and Von Willebrand factor type D(VWD)domains,along with insect-specific motifs such as N-terminal poly-serine tracts,an RNPR cleavage site,a GLCG conserved motif,and C-terminal cysteine residues.The RT-qPCR results show that EonuVg1 and EonuVg2 exhibited low expressions during the early developmental stages(eggs and nymphs),and their expression peaks appreared in the newly emerged female adults.Tissue-specific expression analysis shows that the two genes were highly expressed in the ovary and fat body.After silencing the two genes by RNAi technology,the female duration,oviposition duration and oviposition quantity show a downward trend,but there was no significant difference compared with the control group feeding with ds GFP.The results of imidacloprid stress experiment show that sublethal and higher concentrations of imidacloprid significantly downregulated the expressions of EonuVg1 and EonuVg2 in female adults.This study preliminarily discussed the possible function of EonuVgs in the development and their response to imidacloprid in E.onukii,which provided theoretical basis and data support for further study on the function of Vg genes.关键词
小贯小绿叶蝉/卵黄原蛋白/表达模式/亚致死浓度Key words
Empoasca onukii/vitellogenin/expression pattern/sublethal concentration分类
农业科学引用本文复制引用
潘铖,夏露霞,魏可欣,王梦馨,钮羽群,韩宝瑜..小贯小绿叶蝉卵黄原蛋白基因(EonuVg)克隆和功能研究及其对吡虫啉的响应[J].茶叶科学,2025,45(3):439-453,15.基金项目
国家自然科学基金(32402354、32001910) （32402354、32001910）
