茶叶科学2025，Vol.45Issue(3)：497-508,12.
抹茶对记忆获得障碍模型小鼠的改善及机制分析
Improvement and Mechanism Analysis of Matcha on Mouse Model of Memory Acquisition Impairment
摘要
Abstract
To investigate the ameliorative effect of matcha on the memory acquisition impairment model mice induced by scopolamine and its underlying mechanisms,C57BL/6 female mice were divided into 6 groups,namely the control group,the scopolamine group,the donepezil group(1.50 mg·kg-1),the low-dose matcha group(0.75 g·kg-1),the medium-dose matcha group(1.50 g·kg-1)and the high-dose matcha group(3.00 g·kg-1).On the 31st to 35th days after the injection,the memory acquisition impairment model was established by using scopolamine(5.00 mg·kg-1).The analysis was carried out through methods and techniques such as the Morris water maze test,the step-down test,the dark avoidance experiment,HE staining,enzyme-linked immunosorbent assay(ELISA)and Western blotting.The results demonstrate that matcha could,to a certain extent,improve the learning and memory abilities of the mice with memory acquisition impairment,inhibit the expression of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in the hippocampus tissue,enhance superoxide dismutase(SOD)in the hippocampus tissue,reduce malondialdehyde(MDA)content and glutathione peroxidase(GSH-Px)activity,and upregulate the expressions of Nrf2,SOD,GSH-ST and HO-1 proteins in hippocampal tissue.Meanwhile,it regulated choline metabolism and increased hippocampal choline content,thereby improving the cognitive dysfunction induced by scopolamine in mice.In conclusion,Guizhou-produced matcha plays a positive role in ameliorating memory impairment induced by scopolamine,and its mechanism may be related to enhancing anti-inflammatory activity,alleviating oxidative stress,and regulating neurotransmitters.This study enriched and improved the mechanism of matcha in ameliorating Alzheimer's disease and provided a research foundation and theoretical support for matcha-related health foods.关键词
抹茶/记忆获得障碍模型/氧化应激/神经保护Key words
matcha/memory acquisition impairment model/oxidative stress/neuroprotection分类
农业科技引用本文复制引用
梁增彩,马彩云,郭俣,朱翔,陆涛峰,陈亮,李倩..抹茶对记忆获得障碍模型小鼠的改善及机制分析[J].茶叶科学,2025,45(3):497-508,12.基金项目
贵州省科技计划项目(黔科合基础-[2024]青年048)、贵州省科技计划项目(黔科合基础-ZK[2021]一般156) （黔科合基础-[2024]青年048）
