食品科学2025，Vol.46Issue(13)：1-17,17.DOI:10.7506/spkx1002-6630-20250118-139
微生物细胞工厂合成植物类黄酮:关键酶挖掘及异源生物合成
Production of Plant Flavonoids in Microbial Cell Factories:Key Enzyme Mining and Heterologous Biosynthesis
摘要
Abstract
Plant flavonoids,a class of plant secondary metabolites with diverse physiological activities,hold great application potential in the food,pharmaceutical,cosmetics,and chemical industries.In recent years,the remarkable progress in omics technologies and synthetic biology has significantly advanced the elucidation of the natural biosynthetic pathways of flavonoids and the in-depth functional characterization of their key enzymes.This achievement serves as a crucial theoretical foundation for the construction of microbial cell factories.This review comprehensively outlines the mining strategies for identifying key enzymes in flavonoid biosynthetic pathways,elaborates on the functions of core enzymes and the progress in their engineering modification,and summarizes the latest progress in the microbial synthesis of flavonoid compounds.Moreover,this review analyzes the core bottlenecks in the large-scale production of flavonoids in microbial cell factories from the aspects of heterologous enzyme activity adaptation,metabolic network regulation,and product stability.Finally,it proposes the development direction of interdisciplinary integration and intelligent design,thereby providing theoretical support for the industrialization of flavonoid green biomanufacturing.关键词
类黄酮/微生物细胞工厂/药食同源/关键酶挖掘/路径预测/生物合成Key words
flavonoids/microbial cell factories/medicine and food homology/key enzyme mining/pathway prediction/biosynthesis分类
生物工程引用本文复制引用
胡海涛,吕小妹..微生物细胞工厂合成植物类黄酮:关键酶挖掘及异源生物合成[J].食品科学,2025,46(13):1-17,17.基金项目
"十四五"国家重点研发计划重点专项(2022YFF1100300) （2022YFF1100300）
江苏省重点研发计划项目(BE2022850) （BE2022850）
无锡市太湖创新领军人才项目(1026010241230040) （1026010241230040）
