食品科学2025，Vol.46Issue(14)：207-215,9.DOI:10.7506/spkx1002-6630-20241218-155
动物双歧杆菌NX-6对高脂血症模型斑马鱼的降血脂作用及其机制
Hypolipidemic Effect and Mechanism of Bifidobacterium animalis NX-6 on a Zebrafish Model of Hyperlipidemia
何盈犀 1许欣筑 1林淑娴 1盛雯 1许素哗 1郭丽娜1
作者信息
- 1. 广东省中医院临床营养科,广东 广州 510120
- 折叠
摘要
Abstract
Objective:To analyze the characteristics of Bifidobacterium animalis NX-6 and to explore its lipid-lowering mechanism in the model organism zebrafish using metabolomics.Methods:The growth curve of NX-6 was plotted,and the cell morphology was observed using field emission-scanning electron microscopy(FE-SEM).Its tolerance to simulated gastrointestinal conditions was assessed.Its safety and potential benefits were evaluated at the genetic level through whole-genome sequencing.Untargeted and targeted metabolomics analysis was conducted based on liquid chromatography-mass spectrometry(LC-MS)to identify metabolites produced by NX-6,focusing on key lipid-lowering metabolites.Hyperlipidemia in zebrafish was induced by feeding a high-fat diet,and Oil Red O staining was used to observe lipid accumulation.This study evaluated the lipid-lowering effect and mechanism of NX-6 and its key metabolites.The hypolipidemic effect of the key metabolite indole-3-acetic acid was evaluated on hyperlipidemic zebrafish.Results:Compared with B.animalis BB12,B.animalis NX-6 demonstrated significantly higher tolerance to artificial gastrointestinal fluids.The genome of NX-6 lacked virulence genes but contained abundant lipid and tryptophan metabolism gene clusters.NX-6 significantly reduced total cholesterol and triglyceride levels in hyperlipidemic zebrafish(P<0.001).Indole-3-carboxylic acid and 2-indole ketone were significantly increased in the fermentation supernatant of NX-6 compared with BS medium.Following NX-6 treatment,the content of indole-3-carboxylic acid in zebrafish significantly rose(P<0.001).Indole-3-carboxylic acid notably decreased total cholesterol and triglyceride levels in hyperlipidemic zebrafish(P<0.001).Conclusion:B.animalis NX-6 reduced lipid accumulation in hyperlipidemic zebrafish through its metabolite indole-3-carboxylic acid.关键词
动物双歧杆菌NX-6/色氨酸代谢产物/吲哚-3-甲酸/斑马鱼/降脂作用Key words
Bifidobacterium animalis NX-6/tryptophan metabolites/indole-3-carboxylic acid/zebrafish/lipid-lowering effect分类
生物学引用本文复制引用
何盈犀,许欣筑,林淑娴,盛雯,许素哗,郭丽娜..动物双歧杆菌NX-6对高脂血症模型斑马鱼的降血脂作用及其机制[J].食品科学,2025,46(14):207-215,9.
