食品科学2025，Vol.46Issue(13)：86-93,8.DOI:10.7506/spkx1002-6630-20250107-047
6株野生羊肚菌的分子鉴定及高活力菌株筛选
Molecular Identification and Bioactivity Screening of Six Wild Strains of Morchella sextelata
摘要
Abstract
This study aimed to identify high-quality Morchella strains with strong biological activity and stable fruiting characteristics.We employed single-gene and multi-gene identification techniques for molecular biological identification of six wild strains of Morchella and evaluated their functional traits by measuring various enzyme activities.The activities of antioxidant enzymes catalase(CAT),polyphenol oxidase(PPO)and peroxidase(POD)and malondialdehyde(MDA)content were assayed in liquid-cultured mycelia,and a quantitative assessment of these indicators was performed using the subordinate function method.As a result,we successfully identified an excellent strain suitable for artificial cultivation.Molecular identification confirmed that all six wild Morchella strains belonged to Morchella sextelata,under the Elata Clade group.Morphological observation revealed that the mycelia gradually changed from white to pale yellow,exhibiting dense arrangements with blunt ends and uneven diameters and showing localized spherical distribution characteristics,with a daily growth rate of 0.40-0.45 mm.Mating-type gene analysis indicated that strains LM1,LM3,and LM4 carried both MAT1-1 and MAT1-2 genes,suggesting potential for sexual reproduction.Compared with the two other strains,LM4 exhibited significantly higher PPO,POD,CAT,and 2,3,5-triphenyltetrazolium chloride(TTC)dehydrogenase activities and lower MDA content.The average subordinate function value of LM4 was the highest(0.964 7),highlighting its excellent biological activity and suitability for artificial cultivation.This study provides a novel approach for precise identification and efficient breeding of Morchella germplasm resources.关键词
羊肚菌/分子鉴定/交配型基因/生物量/抗氧化酶活力Key words
Morchella esculenta/strain identification/mating-type genes/biomass/antioxidant enzyme activity分类
生物学引用本文复制引用
向雨萌,吴琦,任海伟,田亚琴,郭晓鹏,刘前逵,王雅钰..6株野生羊肚菌的分子鉴定及高活力菌株筛选[J].食品科学,2025,46(13):86-93,8.基金项目
甘肃省高等学校产业支撑计划项目(2023CYZC-23) （2023CYZC-23）
兰州市科技计划项目(2022-2-94) （2022-2-94）
兰州市青年科技人才创新项目(2024-QN-192) （2024-QN-192）
