食品科学2025，Vol.46Issue(13)：205-213,9.DOI:10.7506/spkx1002-6630-20241225-217
橡木精油的降脂活性及其对秀丽隐杆线虫脂质代谢的影响
Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans
摘要
Abstract
This study investigated the lipid-lowering activity and action mechanism of oak essential oil(OEO).OEO had strong in vitro lipid-lowering activity,and its half-maximal inhibitory concentration(IC50)on pancreatic lipase,cholesterol esterase and α-glucosidase were(0.62±0.05),(0.20±0.04)and(0.63±0.01)mg/mL,respectively.Enzymatic kinetics and fluorescence emission spectroscopy showed that the inhibition type of OEO on pancreatic lipase was reversible non-competitive inhibition,and the quenching type was static quenching.The in vivo lipid-lowering activity of OEO was also assessed using Caenorhabditis elegans as a model organism.It turned out that OEO increased lipid droplets with a diameter of 0-0.6 μm and decreased lipid droplets with a diameter of 1.2-1.8 μm in high-fat C.elegans;20 μg/mL of OEO reduced the fat content and total triglyceride level of high-fat C.elegans by 26.46%and 21.35%,respectively.The results of real-time polymerase chain reaction(PCR)showed that OEO significantly up-regulated the relative expression levels of lipid hydrolysis genes(atgl-1 and hosl-1)and fatty acid beta oxidation genes(acs-2),and significantly down-regulated the relative expression levels of fat synthesis genes(fat-5,fat-6,fat-7 and sbp-1).This study indicates that OEO has lipid-lowering activity,providing data support for its application for the alleviation of obesity.关键词
橡木精油/胰脂肪酶/秀丽隐杆线虫/脂质代谢/基因Key words
oak essential oil/pancreatic lipase/Caenorhabditis elegans/lipid metabolism/gene分类
轻工业引用本文复制引用
魏子杰,段雪娟,王萍萍,陶志华,黎靖宇,张君豪,吴克刚,柴向华,于泓鹏,何东,刘晓丽..橡木精油的降脂活性及其对秀丽隐杆线虫脂质代谢的影响[J].食品科学,2025,46(13):205-213,9.基金项目
河源市科技计划项目(220705101471437) （220705101471437）
广州市科技计划项目(2024E04J0379 （）
202103000078 （）
202206010181) （）
