食品科学2025，Vol.46Issue(13)：232-242,11.DOI:10.7506/spkx1002-6630-20241217-140
基于非靶向代谢组学分析国内不同产地红茶香气代谢物
Non-targeted Metabolomic Analysis of Aroma Metabolites of Black Teas from Different Production Regions in China
摘要
Abstract
Non-targeted metabolomics based on headspace solid-phase microextraction(HS-SPME)coupled to gas chromatography-mass spectrometry(GC-MS),multivariate data analysis and odor activity value(OAV)were integrated to clarify the aroma metabolites in representative black tea samples from different production regions of China and also to determine their key aroma compounds.The results indicated that a total of 140 aroma metabolites were identified in this study,which were classified into 12 types based on their chemical structures,including alcohols,esters,aldehydes,acids,ketones and alkenes.The proportion of alcohols in the total aroma compounds was quite high,ranging from 22.90%to 47.16%.Jiangsu black tea had the highest content of alcohols,followed by Jiangxi,Hubei,Guangdong,Zhejiang,and other regions,while Shandong tea samples had the lowest value.Esters accounted for 2.75%to 26.30%of the total aroma compounds,with the relative content of esters being significantly higher in China Taiwan black tea than tea samples from other regions.Sensory evaluation revealed that all black tea samples shared the common characteristic of sweet aroma,yet each had unique characteristics,presenting a diverse range of aromas,such as medicinal sweet,ripe sweet,roasted sweet,fruity sweet and honey sweet aromas.Black tea samples from Luokeng,Guangdong had a rich almond-like aroma and received the highest score of 96.0 in sensory evaluation.Partial least squares-discriminant analysis(PLS-DA)allowed clear differentiation black tea samples from different regions of China,and 27 differential aroma metabolites were identified based on variable importance in projection(VIP)values>1.0,among which seven including linalool,phenylethanol,phenylacetaldehyde,methyl salicylate,hexanoic acid,1-methylnaphthalene,and geraniol had aroma activity with OAV>1.Methyl salicylate in China Taiwan black tea,as well as phenylacetaldehyde in Guangdong black tea could serve as a marker aroma metabolite to differentiate them from those produced elsewhere.This study offers a scientific basis for identifying the aroma quality of black tea from different regions of China.关键词
非靶向代谢组学/气相色谱-质谱法/香气代谢物/香气活性Key words
non-targeted metabolomics/gas chromatography-mass spectrometry/aroma metabolites/aroma activity分类
轻工业引用本文复制引用
王秋霜,吴华玲,秦丹丹,方开星,姜晓辉,王青,李波,李红建,倪尔冬,潘晨东..基于非靶向代谢组学分析国内不同产地红茶香气代谢物[J].食品科学,2025,46(13):232-242,11.基金项目
广东省重点领域研发计划项目(2020B020220004) （2020B020220004）
现代农业产业技术体系建设专项(CARS-19) （CARS-19）
清远市科技计划项目(2023KJJ008) （2023KJJ008）
广东省驻镇帮镇扶村农村科技特派员项目(KTP20240150 （）
KTP20210176) （）
"广东特支计划"科技创新青年拔尖人才项目(2021TQ06N134) （2021TQ06N134）
江门市科技计划项目(2023760100380008177) （2023760100380008177）
广东省农业农村厅乡村振兴战略专项资金种业振兴项目(2024-NBH-00-001) （2024-NBH-00-001）
